PMID- 10448063
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 262
IP  - 1
DP  - 1999 Aug 19
TI  - CHOP/GADD153 and methionyl-tRNA synthetase (MetRS) genes overlap in a conserved
      region that controls mRNA stability.
PG  - 31-8
AB  - The transcription factor CHOP is involved in the regulation of the cell division 
      cycle and the control of programmed cell death in response to cellular stress.
      CHOP expression has been linked with several forms of cancer. A reciprocal
      translocation between the CHOP and TLS RNA-binding protein gene results in myxoid
      liposarcoma and amplifications of the CHOP gene are associated with solid tumors 
      including several types of sarcomas. Here we report the mapping of the methionyl 
      tRNA synthetase (MetRS) gene to the identical 12q13 locus where the CHOP gene had
      previously been mapped. PCR analysis demonstrates a tail-to-tail overlap of both 
      genes over a 55-bp region. As a result the two mRNAs share a 3' UTR complementary
      sequence allowing an in vivo interaction between the two mRNAs. An AU-rich
      regulatory element (ARE) known to control mRNA stability resides in the
      overlapping sequence. To test for functional significance of the ARE a luciferase
      reporter plasmid containing the 3'UTR of CHOP was constructed. Transfection
      experiments in NIH-3T3 cells show that CHOP 3'UTR confers a significantly lower
      activity than a control reporter or a reporter in which the region overlapping
      the MetRS mRNA is deleted. The conservation of this overlapping of the CHOP and
      MetRS genes and the role of their complementary sequence in the control of mRNA
      stability suggest the existence of a functional link between the expression of
      these two genes.
CI  - Copyright 1999 Academic Press.
FAU - Ubeda, M
AU  - Ubeda M
AD  - Massachusetts General Hospital, Howard Hughes Medical Institute, Boston,
      Massachusetts 02114, USA.
FAU - Schmitt-Ney, M
AU  - Schmitt-Ney M
FAU - Ferrer, J
AU  - Ferrer J
FAU - Habener, J F
AU  - Habener JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (3' Untranslated Regions)
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DDIT3 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Ddit3 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 147336-12-7 (Transcription Factor CHOP)
RN  - EC 6.1.1.10 (Methionine-tRNA Ligase)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - *CCAAT-Enhancer-Binding Proteins
MH  - Chromosomes, Human, Pair 12/genetics
MH  - Conserved Sequence/*genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Expressed Sequence Tags
MH  - Gene Expression Regulation
MH  - Genes, Overlapping/*genetics
MH  - Humans
MH  - Methionine-tRNA Ligase/*genetics
MH  - Mice
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Physical Chromosome Mapping
MH  - RNA, Messenger/genetics/*metabolism
MH  - Regulatory Sequences, Nucleic Acid/*genetics
MH  - Sequence Homology, Nucleic Acid
MH  - Transcription Factor CHOP
MH  - Transcription Factors/*genetics
MH  - Transfection
EDAT- 1999/08/17 00:00
MHDA- 1999/08/17 00:01
CRDT- 1999/08/17 00:00
PHST- 1999/08/17 00:00 [pubmed]
PHST- 1999/08/17 00:01 [medline]
PHST- 1999/08/17 00:00 [entrez]
AID - 10.1006/bbrc.1999.1140 [doi]
AID - S0006-291X(99)91140-3 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 19;262(1):31-8. doi: 10.1006/bbrc.1999.1140.