PMID- 10447878
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20131121
IS  - 0950-382X (Print)
IS  - 0950-382X (Linking)
VI  - 33
IP  - 4
DP  - 1999 Aug
TI  - Sialic acid in the lipopolysaccharide of Haemophilus influenzae: strain
      distribution, influence on serum resistance and structural characterization.
PG  - 679-92
AB  - A survey of Haemophilus influenzae strains indicated that around one-third of
      capsular strains and over two-thirds of non-typeable strains included sialic acid
      in their lipopolysaccharides (LPS). Mutation of the CMP-Neu5Ac synthetase gene
      (siaB) resulted in a sialylation-deficient phenotype. Isogenic pairs, wild type
      and siaB mutant of two non-typeable strains were used to demonstrate that sialic 
      acid influences resistance to the killing effect of normal human serum but has
      little effect on attachment to, or invasion of, cultured human epithelial cells
      or neutrophils. We determine for the first time the site of attachment of sialic 
      acid in the LPS of a non-typeable strain and report that a small proportion of
      glycoforms include two sialic acid residues in a disaccharide unit.
FAU - Hood, D W
AU  - Hood DW
AD  - Molecular Infectious Diseases Group, University of Oxford Department of
      Paediatrics, Institute of Molecular Medicine, John Radcliffe Hospital,
      Headington, Oxford OX3 9DS, UK. dhood@molbiol.ox.ac.uk
FAU - Makepeace, K
AU  - Makepeace K
FAU - Deadman, M E
AU  - Deadman ME
FAU - Rest, R F
AU  - Rest RF
FAU - Thibault, P
AU  - Thibault P
FAU - Martin, A
AU  - Martin A
FAU - Richards, J C
AU  - Richards JC
FAU - Moxon, E R
AU  - Moxon ER
LA  - eng
GR  - AI20897/AI/NIAID NIH HHS/United States
GR  - AI33505/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Mol Microbiol
JT  - Molecular microbiology
JID - 8712028
RN  - 0 (Bacterial Proteins)
RN  - 0 (Disaccharides)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Oligosaccharides)
RN  - EC 2.7.7.43 (N-Acylneuraminate Cytidylyltransferase)
RN  - EC 2.7.7.43 (SiaB protein, Neisseria meningitidis)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
MH  - Bacterial Adhesion/genetics
MH  - Bacterial Proteins/genetics
MH  - Carbohydrate Sequence
MH  - Cell Line
MH  - Disaccharides/chemistry
MH  - Drug Resistance/genetics
MH  - Haemophilus influenzae/enzymology/*genetics
MH  - Lipopolysaccharides/*chemistry
MH  - Molecular Sequence Data
MH  - Mutation
MH  - N-Acetylneuraminic Acid/analysis
MH  - *N-Acylneuraminate Cytidylyltransferase
MH  - Neutrophils
MH  - Oligosaccharides/chemistry
EDAT- 1999/08/14 00:00
MHDA- 1999/08/14 00:01
CRDT- 1999/08/14 00:00
PHST- 1999/08/14 00:00 [pubmed]
PHST- 1999/08/14 00:01 [medline]
PHST- 1999/08/14 00:00 [entrez]
AID - mmi1509 [pii]
PST - ppublish
SO  - Mol Microbiol. 1999 Aug;33(4):679-92.