PMID- 10447508
OWN - NLM
STAT- MEDLINE
DCOM- 19991206
LR  - 20181113
IS  - 1088-9051 (Print)
IS  - 1088-9051 (Linking)
VI  - 9
IP  - 8
DP  - 1999 Aug
TI  - A sequence-ready BAC contig of the GABAA receptor gene cluster
      Gabrg1-Gabra2-Gabrb1 on mouse chromosome 5.
PG  - 732-8
AB  - The type-A receptors for the neurotransmitter GABA (gamma-aminobutyric acid) are 
      ligand-gated chloride channels that mediate postsynaptic inhibition. The
      functional diversity of these receptors comes from the use of a large repertoire 
      of subunits encoded by separate genes, as well as from differences in subunit
      composition of individual receptors. In mammals, a majority of GABA(A) receptor
      subunit genes are located in gene clusters that may be important for their
      regulated expression and function. We have established a high-resolution physical
      map of the cluster of genes encoding GABA(A) receptor subunits alpha2 (Gabra2),
      beta1 (Gabrb1), and gamma(1) (Gabrg1) on mouse chromosome 5. Rat cDNA probes and 
      specific sequence probes for all three GABA(A) receptor subunit genes have been
      used to initiate the construction of a sequence-ready contig of bacterial
      artificial chromosomes (BACs) encompassing this cluster. In the process of contig
      construction clones from 129/Sv and C57BL/6J BAC libraries were isolated. The
      assembled 1.3-Mb contig, consisting of 45 BACs, gives five- to sixfold coverage
      over the gene cluster and provides an average resolution of one marker every 32
      kb. A number of BAC insert ends were sequenced, generating 30 new sequence tag
      sites (STS) in addition to 6 Gabr gene-based and 3 expressed sequence tag
      (EST)-based markers. STSs from, and surrounding, the Gabrg1-Gabra2-Gabrb1 gene
      cluster were mapped in the T31 mouse radiation hybrid panel. The integration of
      the BAC contig with a map of loci ordered by radiation hybrid mapping suggested
      the most likely genomic orientation of this cluster on mouse chromosome 5:
      cen-D5Mit151-Gabrg1-Gabra2-Gabrb1-D5Mit58- tel. This established contig will
      serve as a template for genomic sequencing and for functional analysis of the
      GABA(A) gene cluster on mouse chromosome 5 and the corresponding region on human 
      chromosome 4.
FAU - Lengeling, A
AU  - Lengeling A
AD  - Center for Neurobiology and Behavior, Department of Psychiatry, University of
      Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
FAU - Wiltshire, T
AU  - Wiltshire T
FAU - Otmani, C
AU  - Otmani C
FAU - Bucan, M
AU  - Bucan M
LA  - eng
SI  - GENBANK/AF156490
SI  - GENBANK/AQ589406
SI  - GENBANK/AQ589407
SI  - GENBANK/AQ589408
SI  - GENBANK/AQ589409
SI  - GENBANK/AQ589410
SI  - GENBANK/AQ589411
SI  - GENBANK/AQ589412
SI  - GENBANK/AQ589413
SI  - GENBANK/AQ589414
SI  - GENBANK/AQ589415
SI  - GENBANK/AQ589416
SI  - GENBANK/AQ589417
SI  - GENBANK/AQ589418
SI  - GENBANK/AQ589419
SI  - GENBANK/AQ589420
SI  - GENBANK/AQ589421
SI  - GENBANK/AQ589422
SI  - GENBANK/AQ589423
SI  - GENBANK/AQ589424
SI  - GENBANK/AQ589425
SI  - GENBANK/AQ589426
SI  - GENBANK/AQ589427
SI  - GENBANK/AQ589428
SI  - GENBANK/AQ589429
SI  - GENBANK/AQ589430
SI  - GENBANK/AQ589431
SI  - GENBANK/AQ589432
SI  - GENBANK/AQ589433
SI  - GENBANK/AQ589434
SI  - etc.
GR  - R01 HD028410/HD/NICHD NIH HHS/United States
GR  - HD 28410/HD/NICHD NIH HHS/United States
GR  - MH 57855/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genome Res
JT  - Genome research
JID - 9518021
RN  - 0 (DNA, Bacterial)
RN  - 0 (Genetic Markers)
RN  - 0 (Receptors, GABA-A)
SB  - IM
MH  - Animals
MH  - Chromosomes/*genetics
MH  - Contig Mapping/*methods
MH  - DNA, Bacterial/*analysis
MH  - Genetic Markers
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Multigene Family/*genetics
MH  - Rats
MH  - Receptors, GABA-A/*genetics
PMC - PMC310801
EDAT- 1999/08/14 00:00
MHDA- 1999/08/14 00:01
CRDT- 1999/08/14 00:00
PHST- 1999/08/14 00:00 [pubmed]
PHST- 1999/08/14 00:01 [medline]
PHST- 1999/08/14 00:00 [entrez]
PST - ppublish
SO  - Genome Res. 1999 Aug;9(8):732-8.