PMID- 10446428
OWN - NLM
STAT- MEDLINE
DCOM- 19990917
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1439
IP  - 3
DP  - 1999 Aug 18
TI  - Rat sn-glycerol-3-phosphate acyltransferase: molecular cloning and
      characterization of the cDNA and expressed protein.
PG  - 415-23
AB  - Rat mitochondrial glycerol-3-phosphate acyltransferase (GPAT) cDNA was cloned and
      characterized. We identified a cDNA containing an open reading frame of 828 amino
      acids that had an 89% homology with the coding region of the previously
      characterized mouse mitochondrial GPAT and a predicted amino acid sequence that
      was 96% identical. The rat 5' UTR was only 159 nucleotides, in contrast to the
      926 nucleotide 5' UTR of the mouse cDNA and had an internal deletion of 167
      nucleotides. GPAT was expressed in Sf21 insect cells, and specific inhibitors
      strongly suggest that, like the Escherichia coli GPAT, the recombinant
      mitochondrial GPAT and the mitochondrial GPAT isoform in rat liver contain
      critical serine, histidine, and arginine residues.
FAU - Ganesh Bhat, B
AU  - Ganesh Bhat B
AD  - Departments of Nutrition, Pediatrics, and Medicine, The University of North
      Carolina, Chapel Hill NC 27599-7400, USA.
FAU - Wang, P
AU  - Wang P
FAU - Kim, J H
AU  - Kim JH
FAU - Black, T M
AU  - Black TM
FAU - Lewin, T M
AU  - Lewin TM
FAU - Fiedorek, F T Jr
AU  - Fiedorek FT Jr
FAU - Coleman, R A
AU  - Coleman RA
LA  - eng
SI  - GENBANK/AF021348
GR  - DK56598/DK/NIDDK NIH HHS/United States
GR  - HD08431/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA, Complementary)
RN  - EC 2.3.1.15 (Glycerol-3-Phosphate O-Acyltransferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/genetics
MH  - Glycerol-3-Phosphate O-Acyltransferase/biosynthesis/chemistry/*genetics
MH  - Insecta
MH  - Mitochondria, Liver/enzymology
MH  - Molecular Sequence Data
MH  - Rats
MH  - Sequence Alignment
EDAT- 1999/08/14 10:00
MHDA- 2001/03/28 10:01
CRDT- 1999/08/14 10:00
PHST- 1999/08/14 10:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/08/14 10:00 [entrez]
AID - S1388-1981(99)00103-1 [pii]
AID - 10.1016/s1388-1981(99)00103-1 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Aug 18;1439(3):415-23. doi:
      10.1016/s1388-1981(99)00103-1.