PMID- 10446280
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190910
IS  - 0925-4773 (Print)
IS  - 0925-4773 (Linking)
VI  - 86
IP  - 1-2
DP  - 1999 Aug
TI  - Retroviral promoter-trap insertion into a novel mammalian septin gene expressed
      during mouse neuronal development.
PG  - 183-91
AB  - We have characterized a retroviral promoter-trap insertion into a novel mammalian
      septin gene, Sep3. Its predicted amino acid sequence shares significant homology 
      to that of Saccharomyces cerevisiae CDC3, CDC10, CDC11, CDC12, the Drosophila
      genes Pnut, Sep1, Sep2, and the mammalian genes BH5, CDC10, Nedd5, Diff6, and
      Sep2, which are implicated in cytokinesis and cell polarity. Sep3 encodes a
      protein of 465 amino acids, and contains an evolutionary conserved
      ATP/GTP-binding motif, two coiled-coil domains, and a highly hydrophobic domain
      at the C terminus. Alkaline phosphatase reporter gene expression in transgenic
      embryos was first detected at E8.5 in the neural fold, and high levels of
      expression continued throughout embryogenesis in the neural tube and brain. In
      addition, a low level of transient expression was detected in the somites, gut,
      and branchial arches of mouse embryos. Overall, reporter gene expression
      recapitulated Sep3 mRNA expression during mouse embryogenesis. In adults, Sep3
      transcripts were only detected in the brain and testis. Zoo blot analysis
      revealed that Sep3-related sequences exist in several vertebrate species
      including zebrafish, frog, chicken, mouse and human. Consistent with the
      retroviral insertion into the 3' UTR of the Sep3 gene, no obvious phenotypes
      associated with the promoter trap were detected in transgenic embryos or adult
      mice. In summary, we report the first isolation of a novel full-length Sep3 cDNA 
      and extensive characterization of its expression during mouse embryogenesis and
      in adult tissues.
FAU - Xiong, J W
AU  - Xiong JW
AD  - Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine,
      New York, NY 10029, USA.
FAU - Leahy, A
AU  - Leahy A
FAU - Stuhlmann, H
AU  - Stuhlmann H
LA  - eng
SI  - GENBANK/AF104411
GR  - 1 T32 DK07757-01/DK/NIDDK NIH HHS/United States
GR  - R29 HD-31534/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Mech Dev
JT  - Mechanisms of development
JID - 9101218
RN  - 0 (CDC11 protein, S cerevisiae)
RN  - 0 (CDC12 protein, S cerevisiae)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Fungal Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.1.- (SEPTIN7 protein, human)
RN  - EC 3.6.1.- (Sept2 protein, mouse)
RN  - EC 3.6.1.- (Septins)
RN  - EC 3.6.1.- (septin 3)
SB  - IM
MH  - Alkaline Phosphatase/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/growth & development/metabolism
MH  - Cell Cycle Proteins/genetics
MH  - Central Nervous System/*embryology
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - Cytoskeletal Proteins/genetics
MH  - DNA Transposable Elements
MH  - Female
MH  - Fungal Proteins/genetics
MH  - GTP Phosphohydrolases/genetics
MH  - *GTP-Binding Proteins
MH  - *Gene Expression Regulation, Developmental
MH  - Humans
MH  - Male
MH  - Mammals
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Proteins/*genetics/metabolism
MH  - RNA, Messenger/analysis
MH  - Retroviridae/*genetics
MH  - *Saccharomyces cerevisiae Proteins
MH  - Septins
MH  - Sequence Homology, Amino Acid
MH  - Testis/growth & development/metabolism
EDAT- 1999/08/14 00:00
MHDA- 1999/08/14 00:01
CRDT- 1999/08/14 00:00
PHST- 1999/08/14 00:00 [pubmed]
PHST- 1999/08/14 00:01 [medline]
PHST- 1999/08/14 00:00 [entrez]
AID - S0925477399001136 [pii]
AID - 10.1016/s0925-4773(99)00113-6 [doi]
PST - ppublish
SO  - Mech Dev. 1999 Aug;86(1-2):183-91. doi: 10.1016/s0925-4773(99)00113-6.