PMID- 10446220
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 34
DP  - 1999 Aug 20
TI  - An enhancer element for expression of the Ncx (Enx, Hox11L1) gene in neural
      crest-derived cells.
PG  - 24401-7
AB  - The murine Ncx (Enx, Hox11L1) gene is specifically expressed in a neuronal subset
      of neural crest-derived tissues. In attempts to elucidate the regulatory DNA
      element of the tissue-specific expression, we sequenced the 5'-flanking region of
      the Ncx gene. The transcriptional initiation site was determined at 297
      nucleotides (-297) upstream from the ATG start codon (+1). A retinoic acid
      response element was located on the region between -1163 and -1150. Transient
      transfection assays with the 5'-flanking sequences fused to the luciferase gene
      showed that the region between -1387 and -1368 was crucial for the
      tissue-specific enhancer activity. Furthermore, nuclear proteins extracted from
      neural crest-derived cells such as murine and human neuroblastoma cells bind to
      the DNA region between -1387 and -1368. This DNA element was also conserved in
      the 5'-flanking region of the human NCX gene. Our observations strongly suggest
      that the DNA element (between -1387 and -1368) contributes to tissue-specific
      expression of the Ncx gene in murine and human species.
FAU - Iitsuka, Y
AU  - Iitsuka Y
AD  - Department of Developmental Genetics, Chiba University Graduate School of
      Medicine, Chiba 260-8670, Japan.
FAU - Shimizu, H
AU  - Shimizu H
FAU - Kang, M M
AU  - Kang MM
FAU - Sasagawa, K
AU  - Sasagawa K
FAU - Sekiya, S
AU  - Sekiya S
FAU - Tokuhisa, T
AU  - Tokuhisa T
FAU - Hatano, M
AU  - Hatano M
LA  - eng
SI  - GENBANK/AB008500
SI  - GENBANK/AB008501
SI  - GENBANK/AB008502
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (HOXB1 homeodomain protein)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (TLX2 protein, human)
RN  - 0 (Tlx2 protein, mouse)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Enhancer Elements, Genetic
MH  - Female
MH  - *Gene Expression Regulation
MH  - Homeodomain Proteins/*genetics
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred DBA
MH  - Molecular Sequence Data
MH  - Neural Crest/cytology/*metabolism
MH  - Organ Specificity
MH  - Promoter Regions, Genetic/physiology
MH  - Tretinoin/pharmacology
MH  - Tumor Cells, Cultured
MH  - Up-Regulation
EDAT- 1999/08/14 00:00
MHDA- 1999/08/14 00:01
CRDT- 1999/08/14 00:00
PHST- 1999/08/14 00:00 [pubmed]
PHST- 1999/08/14 00:01 [medline]
PHST- 1999/08/14 00:00 [entrez]
AID - 10.1074/jbc.274.34.24401 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 20;274(34):24401-7. doi: 10.1074/jbc.274.34.24401.