PMID- 10446180
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 34
DP  - 1999 Aug 20
TI  - PIPPin is a brain-specific protein that contains a cold-shock domain and binds
      specifically to H1 degrees and H3.3 mRNAs.
PG  - 24087-93
AB  - During maturation of mammalian brain, variants of both linker (i.e. H1 degrees)
      and core (i.e. H3.3) histone proteins accumulate in nerve cells. As the
      concentration of the corresponding transcripts decreases, in postmitotic cells,
      even if the genes are actively transcribed, it is likely that regulation of
      variant histone expression has relevant post-transcriptional components and that 
      cellular factors affect histone mRNA stability and/or translation. Here we report
      that PIPPin, a protein that is highly enriched in the rat brain and contains a
      cold-shock domain, binds with high specificity to the transcripts that encode H1 
      degrees and H3.3 histone variants. Both mRNAs are bound through the very end of
      their 3'-untranslated region that encompasses the polyadenylation signal.
      Although PIPPin is present both in the cytoplasm and the nucleus of nerve cells, 
      PIPPin-RNA complexes can be obtained only from nuclear extracts. The results of
      two-dimensional electrophoretic analysis suggest that a relevant proportion of
      nuclear PIPPin is more acidic than expected, thus suggesting that its RNA binding
      activity might be modulated by post-translational modifications, such as
      phosphorylation.
FAU - Nastasi, T
AU  - Nastasi T
AD  - Dipartimento di Biologia Cellulare e dello Sviluppo "A. Monroy," viale delle
      Scienze, Parco d'Orleans, 90128 Palermo, Italy.
FAU - Scaturro, M
AU  - Scaturro M
FAU - Bellafiore, M
AU  - Bellafiore M
FAU - Raimondi, L
AU  - Raimondi L
FAU - Beccari, S
AU  - Beccari S
FAU - Cestelli, A
AU  - Cestelli A
FAU - di Liegro, I
AU  - di Liegro I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Histones)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (PIPPin protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - *Brain Chemistry
MH  - Cell Nucleus/chemistry
MH  - Cells, Cultured
MH  - Cold Temperature
MH  - Female
MH  - Histones/*genetics
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/analysis/*metabolism
MH  - RNA, Messenger/*metabolism
MH  - RNA-Binding Proteins/analysis/*metabolism
MH  - Rabbits
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1999/08/14 00:00
MHDA- 1999/08/14 00:01
CRDT- 1999/08/14 00:00
PHST- 1999/08/14 00:00 [pubmed]
PHST- 1999/08/14 00:01 [medline]
PHST- 1999/08/14 00:00 [entrez]
AID - 10.1074/jbc.274.34.24087 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 20;274(34):24087-93. doi: 10.1074/jbc.274.34.24087.