PMID- 10445031
OWN - NLM
STAT- MEDLINE
DCOM- 19990830
LR  - 20131121
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 4
IP  - 1
DP  - 1999 Jul
TI  - NSF N-terminal domain crystal structure: models of NSF function.
PG  - 97-107
AB  - N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential for
      eukaryotic vesicle fusion. Along with SNAP proteins, it disassembles cis-SNARE
      complexes upon ATP hydrolysis, preparing SNAREs for trans complex formation. We
      have determined the crystal structure of the N-terminal domain of NSF (N) to 1.9 
      A resolution. N contains two subdomains which form a groove that is a likely SNAP
      interaction site. Unexpectedly, both N subdomains are structurally similar to
      domains in EF-Tu. Based on this similarity, we propose a model for a large
      conformational change in NSF that drives SNARE complex disassembly.
FAU - Yu, R C
AU  - Yu RC
AD  - Howard Hughes Medical Institute, Yale University, New Haven, Connecticut 06520,
      USA.
FAU - Jahn, R
AU  - Jahn R
FAU - Brunger, A T
AU  - Brunger AT
LA  - eng
SI  - PDB/1QCS
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SNARE Proteins)
RN  - 0 (Selenoproteins)
RN  - 0 (Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins)
RN  - 0 (Vesicular Transport Proteins)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.6.1.- (Peptide Elongation Factor Tu)
RN  - EC 3.6.4.6 (N-Ethylmaleimide-Sensitive Proteins)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - CHO Cells
MH  - Carrier Proteins/*chemistry
MH  - Cricetinae
MH  - Crystallography, X-Ray
MH  - Membrane Proteins/*chemistry/*metabolism
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - N-Ethylmaleimide-Sensitive Proteins
MH  - Peptide Elongation Factor Tu/chemistry
MH  - Protein Conformation
MH  - Protein Structure, Secondary
MH  - Proteins/chemistry
MH  - Recombinant Proteins
MH  - SNARE Proteins
MH  - Selenoproteins
MH  - Sequence Alignment
MH  - Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
MH  - *Vesicular Transport Proteins
EDAT- 1999/08/13 00:00
MHDA- 1999/08/13 00:01
CRDT- 1999/08/13 00:00
PHST- 1999/08/13 00:00 [pubmed]
PHST- 1999/08/13 00:01 [medline]
PHST- 1999/08/13 00:00 [entrez]
AID - S1097-2765(00)80191-4 [pii]
PST - ppublish
SO  - Mol Cell. 1999 Jul;4(1):97-107.