PMID- 10445024
OWN - NLM
STAT- MEDLINE
DCOM- 19990830
LR  - 20190915
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 4
IP  - 1
DP  - 1999 Jul
TI  - SGT1 encodes an essential component of the yeast kinetochore assembly pathway and
      a novel subunit of the SCF ubiquitin ligase complex.
PG  - 21-33
AB  - We have identified SGT1 as a dosage suppressor of skp1-4, a mutation causing
      defects in yeast kinetochore function. Sgt1p physically associates with Skp1p in 
      vivo and in vitro. SGT1 is an essential gene, and different sgt1 conditional
      mutants arrest with either a G1 or G2 DNA content. Genetic and phenotypic
      analyses of sgt1-3 (G2 allele) mutants support an essential role in kinetochore
      function. Sgt1p is required for assembling the yeast kinetochore complex, CBF3,
      via activation of Ctf13p. Sgt1p also associates with SCF (Skp1p/Cdc53p/F box
      protein) ubiquitin ligase. sgt1-5 (G1 allele) mutants are defective in Sic1p
      turnover in vivo and Cln1p ubiquitination in vitro. Human SGT1 rescues an sgt1
      null mutation, suggesting that the function of SGT1 is conserved in evolution.
FAU - Kitagawa, K
AU  - Kitagawa K
AD  - Centre for Molecular Medicine and Therapeutics, University of British Columbia,
      Vancouver, Canada.
FAU - Skowyra, D
AU  - Skowyra D
FAU - Elledge, S J
AU  - Elledge SJ
FAU - Harper, J W
AU  - Harper JW
FAU - Hieter, P
AU  - Hieter P
LA  - eng
SI  - GENBANK/AF132856
SI  - GENBANK/U88830
GR  - AG11085/AG/NIA NIH HHS/United States
GR  - CA16519/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (CEP3 protein, S cerevisiae)
RN  - 0 (CTF13 protein, S cerevisiae)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (S-Phase Kinase-Associated Proteins)
RN  - 0 (SIC1 protein, S cerevisiae)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases)
RN  - EC 6.3.2.- (Peptide Synthases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cell Cycle Proteins/genetics
MH  - Cyclin-Dependent Kinase Inhibitor Proteins
MH  - DNA-Binding Proteins/genetics
MH  - Evolution, Molecular
MH  - Fungal Proteins/genetics
MH  - *Genes, Fungal
MH  - Humans
MH  - Kinetochores/*metabolism
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Nuclear Proteins/genetics
MH  - Peptide Synthases/*genetics/metabolism
MH  - Phenotype
MH  - S-Phase Kinase-Associated Proteins
MH  - SKP Cullin F-Box Protein Ligases
MH  - Saccharomyces cerevisiae/*genetics
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Alignment
MH  - Suppression, Genetic
EDAT- 1999/08/13 00:00
MHDA- 1999/08/13 00:01
CRDT- 1999/08/13 00:00
PHST- 1999/08/13 00:00 [pubmed]
PHST- 1999/08/13 00:01 [medline]
PHST- 1999/08/13 00:00 [entrez]
AID - S1097-2765(00)80184-7 [pii]
AID - 10.1016/s1097-2765(00)80184-7 [doi]
PST - ppublish
SO  - Mol Cell. 1999 Jul;4(1):21-33. doi: 10.1016/s1097-2765(00)80184-7.