PMID- 10444453
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20181105
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 277
IP  - 2
DP  - 1999 Aug
TI  - Molecular cloning, tissue distribution, and functional expression of the human
      Na(+)/H(+) exchanger NHE2.
PG  - G383-90
LID - 10.1152/ajpgi.1999.277.2.G383 [doi]
AB  - In the present report, we describe the cloning of a human colonic cDNA that
      describes the full-length Na(+)/H(+) exchanger (NHE) 2 coding region. The human
      NHE2 (hNHE2) cDNA encodes for a polypeptide of 812 amino acids with a 90% overall
      identity to both rabbit and rat NHE2 isoforms. In comparison with SLC9A2,
      recently reported as the human NHE2, the hNHE2 polypeptide is 115 amino acids
      longer in the NH(2)-terminal end and shows only an 84% DNA nucleotide sequence
      identity. Northern blot analysis revealed that hNHE2 message has an uneven tissue
      distribution, with high levels in the skeletal muscle, colon, and kidney and
      lower levels in the testis, prostate, ovary, and small intestine. Protein
      expression studies with hNHE2 clone showed that a 75-kDa protein was expressed.
      Stable expression of transfected full-length hNHE2 cDNA in Na(+)/H(+)
      exchange-deficient LAP1 cells exhibited Na(+)-dependent pH recovery after an acid
      prepulse that was inhibited by 0.1 mM amiloride. These data indicate that this
      cDNA is the true human NHE2 cDNA and that the encoded protein is capable of
      catalyzing Na(+)/H(+) exchange activity.
FAU - Malakooti, J
AU  - Malakooti J
AD  - Section of Digestive and Liver Diseases, Department of Medicine, The University
      of Illinois at Chicago, Westside Veterans Affairs Medical Center, Chicago,
      Illinois 60612, USA. malakoot@uic.edu
FAU - Dahdal, R Y
AU  - Dahdal RY
FAU - Schmidt, L
AU  - Schmidt L
FAU - Layden, T J
AU  - Layden TJ
FAU - Dudeja, P K
AU  - Dudeja PK
FAU - Ramaswamy, K
AU  - Ramaswamy K
LA  - eng
GR  - DK-33349/DK/NIDDK NIH HHS/United States
GR  - DK-54016/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (DNA, Complementary)
RN  - 0 (Sodium-Hydrogen Exchanger 3)
RN  - 0 (Sodium-Hydrogen Exchangers)
SB  - IM
MH  - Amino Acid Sequence/genetics
MH  - Animals
MH  - Base Sequence/genetics
MH  - Cell Line
MH  - *Cloning, Molecular
MH  - DNA, Complementary/genetics/physiology
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Sodium-Hydrogen Exchanger 3
MH  - Sodium-Hydrogen Exchangers/*genetics/*metabolism
MH  - Tissue Distribution
EDAT- 1999/08/13 00:00
MHDA- 1999/08/13 00:01
CRDT- 1999/08/13 00:00
PHST- 1999/08/13 00:00 [pubmed]
PHST- 1999/08/13 00:01 [medline]
PHST- 1999/08/13 00:00 [entrez]
AID - 10.1152/ajpgi.1999.277.2.G383 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 Aug;277(2):G383-90. doi: 10.1152/ajpgi.1999.277.2.G383.