PMID- 10444329
OWN - NLM
STAT- MEDLINE
DCOM- 19991130
LR  - 20071114
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 59
IP  - 3
DP  - 1999 Aug 1
TI  - Retrotransposition of glycerol kinase-related genes from the X chromosome to
      autosomes: functional and evolutionary aspects.
PG  - 282-90
AB  - Glycerol kinase catalyzes the metabolism of endogenously derived and dietary
      glycerol. GyK is a member of a small group of kinases termed ambiquitous enzymes,
      which are found either in the cytosol or as membrane-bound complexes associated
      with the voltage-dependent anion channel of the mitochondrial outer membrane. In 
      Homo sapiens, the GyK gene family consists of an X-encoded locus and several
      X-linked and autosomal intronless retroposons, which, apparently, comprise both
      functional genes and processed pseudogenes. To study the role of the autosomal
      genes in mammalian physiology, we have isolated two murine GyK-like genes,
      determined their structures and chromosomal locations, and examined their
      functions. These sequences are intronless retroposons, which appear to be
      paralogues of the X-encoded, brain-specific GyK isoform and are expressed only in
      the testes. Though both retrotransposition events appear to have occurred prior
      to the primate-rodent divergence of some 65-80 million years ago, only one of the
      retrotransposed murine gene sequences, based upon its chromosomal location, is
      conserved with modern H. sapiens. To test the hypothesis that the murine GyK-like
      genes encode functional GyK activity, transient transfection of the gene
      sequences into COS7 cells was carried out. While in vitro translation confirmed
      that the transcripts could direct the synthesis of proteins of the appropriate
      size, no GyK activity was detected. Such data suggest that the autosomal GyK-like
      genes have evolved novel, testis-specific functions. A comparison of the human
      and mouse GyK-like gene sequences demonstrates the evolutionary relationships
      between each autosomal isoform and its corresponding X-linked ancestral locus.
CI  - Copyright 1999 Academic Press.
FAU - Pan, Y
AU  - Pan Y
AD  - Department of Molecular and Human Genetics, Department of Pediatrics, Baylor
      College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
FAU - Decker, W K
AU  - Decker WK
FAU - Huq, A H
AU  - Huq AH
FAU - Craigen, W J
AU  - Craigen WJ
LA  - eng
SI  - GENBANK/AF117733
SI  - GENBANK/AF117734
GR  - 1P30-HD27823/HD/NICHD NIH HHS/United States
GR  - 2P30-HD24064/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA, Complementary)
RN  - 0 (Retroelements)
RN  - EC 2.7.1.30 (Glycerol Kinase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA, Complementary
MH  - Eukaryotic Cells
MH  - *Evolution, Molecular
MH  - Gene Expression
MH  - Glycerol Kinase/biosynthesis/*genetics
MH  - Humans
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Retroelements
MH  - Testis/enzymology
MH  - *X Chromosome
EDAT- 1999/08/13 00:00
MHDA- 1999/08/13 00:01
CRDT- 1999/08/13 00:00
PHST- 1999/08/13 00:00 [pubmed]
PHST- 1999/08/13 00:01 [medline]
PHST- 1999/08/13 00:00 [entrez]
AID - 10.1006/geno.1999.5874 [doi]
AID - S0888-7543(99)95874-3 [pii]
PST - ppublish
SO  - Genomics. 1999 Aug 1;59(3):282-90. doi: 10.1006/geno.1999.5874.