PMID- 10444327
OWN - NLM
STAT- MEDLINE
DCOM- 19991130
LR  - 20190402
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 59
IP  - 3
DP  - 1999 Aug 1
TI  - Identification, functional characterization, and chromosomal localization of
      USP15, a novel human ubiquitin-specific protease related to the UNP oncoprotein, 
      and a systematic nomenclature for human ubiquitin-specific proteases.
PG  - 264-74
AB  - We have identified a novel gene, USP15, encoding a human ubiquitin-specific
      protease (USP). The USP15 protein consists of 952 amino acids with a predicted
      molecular mass of 109.2 kDa and contains the highly conserved Cys and His boxes
      present in all members of the UBP family of deubiquitinating enzymes. USP15
      shares 60.5% sequence identity and 76% sequence similarity with the human homolog
      (UNP/Unph/USP4) of the mouse Unp proto-oncogene. Recombinant USP15 demonstrated
      ubiquitin-specific protease activity against engineered linear fusions of
      ubiquitin to beta-galactosidase and glutathione S-transferase. USP15 can also
      cleave the ubiquitin-proline bond, a property previously unique to Unp/UNP.
      Chromosomal mapping by fluorescence in situ hybridization and radiation hybrid
      analyses localized the USP15 gene to chromosome band 12q14, a different location 
      than that of UNP (3p21.3). Analysis of expressed sequence tag databases reveals
      evidence of alternate polyadenylation sites in the USP15 gene and also indicates 
      that the gene may possess an exon/intron structure similar to that of the Unp
      gene, suggesting they have descended from a common ancestor. A systematic
      nomenclature for the human USPs is proposed.
CI  - Copyright 1999 Academic Press.
FAU - Baker, R T
AU  - Baker RT
AD  - Division of Molecular Medicine, Australian National University, Canberra, ACT,
      2601, Australia. Rohan.Baker@anu.edu.au
FAU - Wang, X W
AU  - Wang XW
FAU - Woollatt, E
AU  - Woollatt E
FAU - White, J A
AU  - White JA
FAU - Sutherland, G R
AU  - Sutherland GR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Avian Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MAFB protein, human)
RN  - 0 (MafB Transcription Factor)
RN  - 0 (Mafb protein, mouse)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (USP4 protein, human)
RN  - 0 (Ubiquitins)
RN  - 0 (Usp4 protein, mouse)
RN  - 9DLQ4CIU6V (Proline)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.19.12 (USP15 protein, human)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
RN  - EC 3.4.19.12 (Ubiquitin-Specific Proteases)
RN  - EC 3.4.19.12 (Usp15 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Avian Proteins
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 12
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - *DNA-Binding Proteins
MH  - Endopeptidases/*genetics/metabolism
MH  - Humans
MH  - MafB Transcription Factor
MH  - Mice
MH  - Molecular Sequence Data
MH  - Oncogene Proteins/classification/*genetics
MH  - Proline/metabolism
MH  - Proto-Oncogene Proteins
MH  - Sequence Homology, Amino Acid
MH  - Terminology as Topic
MH  - Trans-Activators/*genetics
MH  - *Transcription Factors
MH  - Ubiquitin Thiolesterase
MH  - Ubiquitin-Specific Proteases
MH  - Ubiquitins/*metabolism
EDAT- 1999/08/13 00:00
MHDA- 1999/08/13 00:01
CRDT- 1999/08/13 00:00
PHST- 1999/08/13 00:00 [pubmed]
PHST- 1999/08/13 00:01 [medline]
PHST- 1999/08/13 00:00 [entrez]
AID - 10.1006/geno.1999.5879 [doi]
AID - S0888-7543(99)95879-2 [pii]
PST - ppublish
SO  - Genomics. 1999 Aug 1;59(3):264-74. doi: 10.1006/geno.1999.5879.