PMID- 10444069
OWN - NLM
STAT- MEDLINE
DCOM- 19990901
LR  - 20191210
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 146
IP  - 3
DP  - 1999 Aug 9
TI  - Two compartments for insulin-stimulated exocytosis in 3T3-L1 adipocytes defined
      by endogenous ACRP30 and GLUT4.
PG  - 609-20
AB  - Insulin stimulates adipose cells both to secrete proteins and to translocate the 
      GLUT4 glucose transporter from an intracellular compartment to the plasma
      membrane. We demonstrate that whereas insulin stimulation of 3T3-L1 adipocytes
      has no effect on secretion of the alpha3 chain of type VI collagen, secretion of 
      the protein hormone adipocyte complement related protein of 30 kD (ACRP30) is
      markedly enhanced. Like GLUT4, regulated exocytosis of ACRP30 appears to require 
      phosphatidylinositol-3-kinase activity, since insulin-stimulated ACRP30 secretion
      is blocked by pharmacologic inhibitors of this enzyme. Thus, 3T3-L1 adipocytes
      possess a regulated secretory compartment containing ACRP30. Whether GLUT4
      recycles to such a compartment has been controversial. We present deconvolution
      immunofluorescence microscopy data demonstrating that the subcellular
      distributions of ACRP30 and GLUT4 are distinct and nonoverlapping; in contrast,
      those of GLUT4 and the transferrin receptor overlap. Together with supporting
      evidence that GLUT4 does not recycle to a secretory compartment via the
      trans-Golgi network, we conclude that there are at least two compartments that
      undergo insulin-stimulated exocytosis in 3T3-L1 adipocytes: one for ACRP30
      secretion and one for GLUT4 translocation.
FAU - Bogan, J S
AU  - Bogan JS
AD  - Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142-1479,
      USA.
FAU - Lodish, H F
AU  - Lodish HF
LA  - eng
GR  - DK02371/DK/NIDDK NIH HHS/United States
GR  - DK47618/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Adaptor Protein Complex gamma Subunits)
RN  - 0 (Adiponectin)
RN  - 0 (Blood Proteins)
RN  - 0 (Coatomer Protein)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Insulin)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Transferrin)
RN  - 0 (Slc2a4 protein, mouse)
RN  - 0 (endoplasmin)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - 3T3 Cells
MH  - Adaptor Protein Complex gamma Subunits
MH  - Adipocytes/*cytology/drug effects/metabolism
MH  - Adiponectin
MH  - Animals
MH  - Blood Proteins/*analysis/metabolism
MH  - Coatomer Protein
MH  - Collagen/metabolism
MH  - Endoplasmic Reticulum/chemistry/metabolism
MH  - Exocytosis/*drug effects
MH  - Fluorescent Antibody Technique
MH  - Glucose Transporter Type 4
MH  - Golgi Apparatus/chemistry
MH  - Insulin/*pharmacology
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Membrane Glycoproteins/analysis
MH  - Membrane Proteins/analysis
MH  - Mice
MH  - Microtubule-Associated Proteins/analysis
MH  - Monosaccharide Transport Proteins/*analysis/metabolism
MH  - *Muscle Proteins
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - *Proteins
MH  - Receptors, Transferrin/analysis
PMC - PMC2150549
EDAT- 1999/08/12 00:00
MHDA- 1999/08/12 00:01
CRDT- 1999/08/12 00:00
PHST- 1999/08/12 00:00 [pubmed]
PHST- 1999/08/12 00:01 [medline]
PHST- 1999/08/12 00:00 [entrez]
AID - 10.1083/jcb.146.3.609 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Aug 9;146(3):609-20. doi: 10.1083/jcb.146.3.609.