PMID- 10441733
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20220223
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 10
IP  - 9
DP  - 1999 Sep
TI  - Genomic organization and expression analysis of mouse kynurenine aminotransferase
      II, a possible factor in the pathophysiology of Huntington's disease.
PG  - 845-52
AB  - Decreased levels of the endogenous neuroprotectant kynurenic acid (KYNA) have
      been observed in the brain of Huntington's Disease (HD) patients and may be
      related to neuronal loss in this disorder. This reduction may be caused by a
      dysfunction of kynurenine aminotransferase II (KAT II), the major enzyme
      responsible for the synthesis of KYNA in the brain. Towards understanding the
      role of KAT II in HD, we isolated and characterized the cDNA sequence and
      determined the genomic organization of mouse KAT II (mKat-2). The full length
      mKat-2 cDNA is 1812 bp, encoding 425 amino acids, and shares 89.9% amino acid
      similarity with the rat Kat-2 sequence. The gene for mKat-2 is composed of 13
      exons divided by 12 intronic sequences. Northern blot analysis demonstrated that 
      mKat-2 mRNA is mainly expressed in kidney and liver. RT-PCR showed mKat-2
      expression in the brain starting from at least d11 of embryonic development. An
      alternative isoform mKat-2beta, derived from the usage of novel exons, shows a
      different expression pattern from mKat-2. Western blot analysis of various mouse 
      tissues shows a 40-kDa protein in brain, heart, kidney, and liver. In the kidney 
      and liver an additional 45-kDa isoform was detected. Use of the BSS chromosomal
      mapping panel from The Jackson Laboratory indicates that the mKat-2 gene
      co-segregates with polymorphic markers D8Mit129 and D8Mit128 on mouse Chr 8.
      Knowledge of the genomic organization, the isoform tissue-specific expression
      patterns, the chromosomal localization of mKat-2, and the reagents generated
      here, will provide the tools for further studies and allow generation and
      characterization of mice that are nullizygous for mKat-2.
FAU - Yu, P
AU  - Yu P
AD  - Genetics and Molecular Biology Branch, National Human Genome Research Institute, 
      Building 49, Room 3A26, 49 Convent Drive MSC 4442, National Institutes of Health,
      Bethesda, Maryland 20892-4442, USA.
FAU - Mosbrook, D M
AU  - Mosbrook DM
FAU - Tagle, D A
AU  - Tagle DA
LA  - eng
SI  - GENBANK/AF072376
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 2.6.1.- (Transaminases)
RN  - EC 2.6.1.64 (glutamine - phenylpyruvate transaminase)
RN  - EC 2.6.1.7 (kynurenine-oxoglutarate transaminase)
RN  - EC 4.- (Lyases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/enzymology
MH  - Chromosome Mapping
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Exons
MH  - Gene Expression
MH  - Genome
MH  - Humans
MH  - Huntington Disease/*enzymology/*genetics
MH  - Introns
MH  - Isoenzymes/*genetics
MH  - Kidney/enzymology
MH  - Liver/enzymology
MH  - *Lyases
MH  - Mice
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - Transaminases/*genetics
EDAT- 1999/08/12 00:00
MHDA- 1999/08/12 00:01
CRDT- 1999/08/12 00:00
PHST- 1999/08/12 00:00 [pubmed]
PHST- 1999/08/12 00:01 [medline]
PHST- 1999/08/12 00:00 [entrez]
AID - MG99-653 [pii]
AID - 10.1007/s003359901102 [doi]
PST - ppublish
SO  - Mamm Genome. 1999 Sep;10(9):845-52. doi: 10.1007/s003359901102.