PMID- 10441506
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 261
IP  - 3
DP  - 1999 Aug 11
TI  - Identification and characterization of PKNbeta, a novel isoform of protein kinase
      PKN: expression and arachidonic acid dependency are different from those of
      PKNalpha.
PG  - 808-14
AB  - The cDNA clone encoding a novel isoform of protein kinase PKN, termed PKNbeta,
      was isolated from a HeLa cDNA library. PKNbeta had high sequence homology with
      PKNalpha, originally isolated PKN, especially in the repeats of charged amino
      acid-rich region with leucine-zipper like sequences (CZ region/HR1), in the
      carboxyl-terminal catalytic domain, and in approximately 130 amino acid stretch
      (D region/HR2), located between CZ region/HR1 and the catalytic domain. However, 
      the amino acid sequence of PKNbeta differed from that of PKNalpha in the region
      immediately amino-terminal to the catalytic domain, which contained two distinct 
      proline-rich sequences consistent with the class II consensus sequence, PXXPXR,
      for binding to SH3 domain. Distribution of PKNbeta differed from that of PKNalpha
      in the following two respects: (1) Northern blotting indicated that PKNbeta mRNA 
      could not be detected in human adult tissues, but was expressed abundantly in
      human cancer cell lines; (2) immunochemical analysis indicated that PKNbeta
      localized in nucleus and perinuclear Golgi apparatus, and was almost absent in
      cytoplasmic region in NIH3T3 cells. Recombinant PKNbeta expressed in COS7 cells
      displayed autophosphorylation and peptide kinase activity, but was found to be
      significantly less responsive to arachidonic acid than PKNalpha. The
      identification of this novel isoform underscores the diversity of PKN signaling
      pathway.
CI  - Copyright 1999 Academic Press.
FAU - Oishi, K
AU  - Oishi K
AD  - Graduate School of Science and Technology, Faculty of Science, Kobe, 657-8501,
      Japan.
FAU - Mukai, H
AU  - Mukai H
FAU - Shibata, H
AU  - Shibata H
FAU - Takahashi, M
AU  - Takahashi M
FAU - Ona, Y
AU  - Ona Y
LA  - eng
SI  - GENBANK/AB019692
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.1.- (protein kinase N)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Arachidonic Acid/pharmacology
MH  - Base Sequence
MH  - Binding Sites
MH  - COS Cells
MH  - Cell Nucleus/enzymology
MH  - Consensus Sequence
MH  - DNA, Complementary/isolation & purification
MH  - HeLa Cells
MH  - Humans
MH  - Isoenzymes/chemistry/*genetics/*metabolism
MH  - Leucine Zippers
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Protein Kinase C/chemistry/*genetics/*metabolism
MH  - RNA, Messenger/analysis
MH  - Sequence Homology
MH  - Tumor Cells, Cultured
EDAT- 1999/08/12 00:00
MHDA- 1999/08/12 00:01
CRDT- 1999/08/12 00:00
PHST- 1999/08/12 00:00 [pubmed]
PHST- 1999/08/12 00:01 [medline]
PHST- 1999/08/12 00:00 [entrez]
AID - 10.1006/bbrc.1999.1116 [doi]
AID - S0006-291X(99)91116-6 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 11;261(3):808-14. doi:
      10.1006/bbrc.1999.1116.