PMID- 10441476
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20200311
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 261
IP  - 3
DP  - 1999 Aug 11
TI  - Isolation and identification of melanin-concentrating hormone as the endogenous
      ligand of the SLC-1 receptor.
PG  - 622-6
AB  - Melanin-concentrating hormone (MCH), which is an orexigenic peptide, was isolated
      and identified as the endogenous ligand of the SLC-1 receptor. We established a
      CHO cell line expressing the rat SLC-1 receptor to search for its endogenous
      ligand. The extract of rat whole brain showed inhibition of intracellular
      forskolin-induced cAMP accumulation in rat SLC-1-expressing CHO cells and was
      purified. Using HPLC purification, we isolated and identified MCH as the
      endogenous ligand of the SLC-1 receptor. The authentic MCH demonstrated a
      dose-dependent inhibitory effect on cAMP accumulation in forskolin-stimulated rat
      and human SLC-1-expressing CHO cells with an EC(50) value of 0.2 nM for both the 
      rat and human SLC-1 receptors. This is the first description of the functional
      receptor for MCH.
CI  - Copyright 1999 Academic Press.
FAU - Shimomura, Y
AU  - Shimomura Y
AD  - Pharmaceutical Discovery Research Division, Takeda Chemical Industries, Ltd.,
      Wadai 10, Tsukuba, Ibaraki, 300-4293, Japan.
FAU - Mori, M
AU  - Mori M
FAU - Sugo, T
AU  - Sugo T
FAU - Ishibashi, Y
AU  - Ishibashi Y
FAU - Abe, M
AU  - Abe M
FAU - Kurokawa, T
AU  - Kurokawa T
FAU - Onda, H
AU  - Onda H
FAU - Nishimura, O
AU  - Nishimura O
FAU - Sumino, Y
AU  - Sumino Y
FAU - Fujino, M
AU  - Fujino M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Hypothalamic Hormones)
RN  - 0 (Ligands)
RN  - 0 (MCHR1 protein, human)
RN  - 0 (MCHR1 protein, rat)
RN  - 0 (Melanins)
RN  - 0 (Pituitary Hormones)
RN  - 0 (Receptors, Somatostatin)
RN  - 0 (Recombinant Proteins)
RN  - 1F7A44V6OU (Colforsin)
RN  - 67382-96-1 (melanin-concentrating hormone)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain Chemistry
MH  - CHO Cells
MH  - Chromatography, High Pressure Liquid
MH  - Colforsin/pharmacology
MH  - Cricetinae
MH  - Cyclic AMP/metabolism
MH  - GTP-Binding Proteins/chemistry/genetics/*metabolism
MH  - Gene Expression
MH  - Humans
MH  - Hypothalamic Hormones/*isolation & purification/*metabolism
MH  - Ligands
MH  - Male
MH  - Melanins/*isolation & purification/*metabolism
MH  - Molecular Sequence Data
MH  - Pituitary Hormones/*isolation & purification/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Somatostatin/chemistry/genetics/*metabolism
MH  - Recombinant Proteins/metabolism
EDAT- 1999/08/12 00:00
MHDA- 1999/08/12 00:01
CRDT- 1999/08/12 00:00
PHST- 1999/08/12 00:00 [pubmed]
PHST- 1999/08/12 00:01 [medline]
PHST- 1999/08/12 00:00 [entrez]
AID - 10.1006/bbrc.1999.1104 [doi]
AID - S0006-291X(99)91104-X [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 11;261(3):622-6. doi:
      10.1006/bbrc.1999.1104.