PMID- 10441475
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 261
IP  - 3
DP  - 1999 Aug 11
TI  - Human ZHX1: cloning, chromosomal location, and interaction with transcription
      factor NF-Y.
PG  - 614-21
AB  - NF-YA, B, and C comprise the heterotrimeric transcription factor known as nuclear
      factor Y (NF-Y) or CCAAT-binding protein (CBF). NF-Y binds many CCAAT and Y box
      (an inverted CCAAT box, ATTGG) elements. Mutations of these elements that disrupt
      the binding of NF-Y result in decreased transcription from various
      tissue-specific and inducible promoters. We employed a yeast two-hybrid system to
      screen a human liver cDNA library in an effort to isolate proteins that interact 
      with NF-Y and that may play a role in tissue-specific or hormone-inducible
      promoter activity. Using a fragment of the NF-YA subunit as bait we isolated a
      cDNA that encodes most of the open reading frame of the human zinc fingers and
      homeobox 1 (ZHX1) protein. The complete open reading frame was subsequently
      isolated and found to encode a protein of 873 amino acids that contains two zinc 
      fingers and five homeodomain motifs. Northern blot analysis of poly(A)(+) RNA
      isolated from various tissues revealed two major ZHX1 transcripts of about 4.5
      and 5 kilobases. Both transcripts were expressed ubiquitously, although the
      5-kilobase transcript is of greater abundance in most tissues examined. The human
      ZHX1 gene is located on chromosome 8q, between markers CHCL.GATA50B06 and CHLC.
      GATA7G07.
CI  - Copyright 1999 Academic Press.
FAU - Yamada, K
AU  - Yamada K
AD  - Department of Molecular Physiology and Biophysics, Vanderbilt University School
      of Medicine, 707 Light Hall, Nashville, Tennessee, 37232-0615, USA.
FAU - Printz, R L
AU  - Printz RL
FAU - Osawa, H
AU  - Osawa H
FAU - Granner, D K
AU  - Granner DK
LA  - eng
SI  - GENBANK/AF106862
GR  - CA68485/CA/NCI NIH HHS/United States
GR  - DK20593/DK/NIDDK NIH HHS/United States
GR  - DK46867/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (ZHX1 protein, human)
RN  - 0 (Zhx1 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - CCAAT-Enhancer-Binding Proteins
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 8
MH  - DNA, Complementary/chemistry/isolation & purification
MH  - DNA-Binding Proteins/*metabolism
MH  - Gene Library
MH  - Homeodomain Proteins/chemistry/*genetics/metabolism
MH  - Humans
MH  - Liver/chemistry
MH  - Mice
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Polymerase Chain Reaction
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/analysis
MH  - Sequence Analysis
MH  - Tissue Distribution
MH  - Transcription Factors/chemistry/*genetics/metabolism
MH  - Zinc Fingers
EDAT- 1999/08/12 00:00
MHDA- 1999/08/12 00:01
CRDT- 1999/08/12 00:00
PHST- 1999/08/12 00:00 [pubmed]
PHST- 1999/08/12 00:01 [medline]
PHST- 1999/08/12 00:00 [entrez]
AID - 10.1006/bbrc.1999.1087 [doi]
AID - S0006-291X(99)91087-2 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 11;261(3):614-21. doi:
      10.1006/bbrc.1999.1087.