PMID- 10441464
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 261
IP  - 3
DP  - 1999 Aug 11
TI  - Characterization and phosphorylation of PDE10A2, a novel alternative splice
      variant of human phosphodiesterase that hydrolyzes cAMP and cGMP.
PG  - 551-7
AB  - We have isolated a novel alternative splice variant of human cAMP- and
      cGMP-hydrolyzing phosphodiesterase (PDE10A2) from human fetal lung. The
      N-terminal sequence of human PDE10A2 differed from that of human PDE10A1 reported
      previously. PDE10A1 and PDE10A2 expressed in COS-7 cells have cGMP K(m) values of
      14 and 13 microM, low cAMP K(m) values of 0.28 and 0.22 microM, and high cAMP
      K(m) values of 0.96 and 1.1 microM, respectively, at high concentrations of cGMP 
      and cAMP. PCR analysis demonstrated that both PDE10A1 and PDE10A2 transcripts are
      present in various human tissues and that PDE10A2 transcripts are a major form in
      some human tissues. The unique N-terminus of PDE10A2 has a putative
      phosphorylation site by cAMP-dependent protein kinase (cAK), but PDE10A1 does
      not. The recombinant PDE10A2 protein is preferentially phosphorylated by cAK,
      although the recombinant PDE10A1 protein is not phosphorylated by cAK.
CI  - Copyright 1999 Academic Press.
FAU - Kotera, J
AU  - Kotera J
AD  - Discovery Research Laboratory, Tanabe Seiyaku Co. Ltd., 2-50, Kawagishi-2-chome, 
      Toda, Saitama, 335-8505, Japan.
FAU - Fujishige, K
AU  - Fujishige K
FAU - Yuasa, K
AU  - Yuasa K
FAU - Omori, K
AU  - Omori K
LA  - eng
SI  - GENBANK/AB026816
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 3.1.4.- (PDE10A protein, human)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - COS Cells
MH  - Cyclic AMP/*metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Cyclic GMP/*metabolism
MH  - Gene Expression
MH  - *Genetic Variation
MH  - Humans
MH  - Hydrolysis
MH  - Lung/embryology/enzymology
MH  - Molecular Sequence Data
MH  - Phosphoric Diester Hydrolases/chemistry/genetics/*metabolism
MH  - Phosphorylation
MH  - RNA, Messenger/analysis
MH  - Recombinant Proteins/metabolism
MH  - Tissue Distribution
MH  - Transfection
EDAT- 1999/08/12 00:00
MHDA- 1999/08/12 00:01
CRDT- 1999/08/12 00:00
PHST- 1999/08/12 00:00 [pubmed]
PHST- 1999/08/12 00:01 [medline]
PHST- 1999/08/12 00:00 [entrez]
AID - 10.1006/bbrc.1999.1013 [doi]
AID - S0006-291X(99)91013-6 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 11;261(3):551-7. doi:
      10.1006/bbrc.1999.1013.