PMID- 10441236
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20061115
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 412
IP  - 3
DP  - 1999 Sep 27
TI  - Pigment epithelium-derived factor promotes the survival and differentiation of
      developing spinal motor neurons.
PG  - 506-14
AB  - Pigment epithelium-derived factor (PEDF) is a member of the serine protease
      inhibitor (serpin) superfamily that has been shown previously to promote the
      survival and/or differentiation of rat cerebellar granule neurons and human
      retinoblastoma cells in vitro. However, in contrast to most serpins, PEDF has no 
      inhibitory activity against any known proteases, and its described biological
      activities do not appear to require the serpin-reactive loop located toward the
      carboxy end of the polypeptide. Because another serpin, protease nexin-1, has
      been shown to promote the in vivo survival and growth of motor neurons, the
      authors investigated the potential neurotrophic effects of PEDF on spinal cord
      motor neurons in highly enriched cultures and in vivo after injury. Here, it is
      shown that native bovine and recombinant human PEDF promoted the survival and
      differentiation (neurite outgrowth) of embryonic chick spinal cord motor neurons 
      in vitro in a dose-dependent manner. A truncated form of PEDF that lacks
      approximately 62% of the carboxy end of the polypeptide comprising the homologous
      serpin-reactive loop also exhibited neurotrophic activities similar to those of
      the full-length protein. Furthermore, the data here showed that PEDF was
      transported retrogradely and prevented the death and atrophy of spinal motor
      neurons in the developing neonatal mouse after axotomy. These results indicate
      that PEDF exerts trophic effects on motor neurons, and, together with previous
      reports, these findings suggest that this protein may be useful as a
      pharmacologic agent to promote the development and maintenance of motor neurons. 
      J. Comp. Neurol. 412:506-514, 1999. Published 1999 Wiley-Liss, Inc.
FAU - Houenou, L J
AU  - Houenou LJ
AD  - Department of Neurobiology and Anatomy, Wake Forest University School of
      Medicine, Winston-Salem, North Carolina 27157, USA. lhouenou@wfubmc.edu
FAU - D'Costa, A P
AU  - D'Costa AP
FAU - Li, L
AU  - Li L
FAU - Turgeon, V L
AU  - Turgeon VL
FAU - Enyadike, C
AU  - Enyadike C
FAU - Alberdi, E
AU  - Alberdi E
FAU - Becerra, S P
AU  - Becerra SP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Eye Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Serpins)
RN  - 0 (pigment epithelium-derived factor)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Axotomy
MH  - Biological Transport
MH  - Cattle
MH  - Cell Differentiation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Dose-Response Relationship, Drug
MH  - *Eye Proteins
MH  - Humans
MH  - Mice
MH  - Motor Neurons/cytology/*drug effects
MH  - Nerve Growth Factors/*pharmacology
MH  - Neurites/drug effects
MH  - Proteins/*pharmacology
MH  - Recombinant Proteins/pharmacology
MH  - Serpins/*pharmacology
MH  - Spinal Cord/*drug effects/embryology/growth & development
EDAT- 1999/08/11 00:00
MHDA- 1999/08/11 00:01
CRDT- 1999/08/11 00:00
PHST- 1999/08/11 00:00 [pubmed]
PHST- 1999/08/11 00:01 [medline]
PHST- 1999/08/11 00:00 [entrez]
AID - 10.1002/(SICI)1096-9861(19990927)412:3<506::AID-CNE9>3.0.CO;2-E [pii]
PST - ppublish
SO  - J Comp Neurol. 1999 Sep 27;412(3):506-14.