PMID- 10438970
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20071115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 163
IP  - 4
DP  - 1999 Aug 15
TI  - Sequence polymorphisms in the chemokines Scya1 (TCA-3), Scya2 (monocyte
      chemoattractant protein (MCP)-1), and Scya12 (MCP-5) are candidates for eae7, a
      locus controlling susceptibility to monophasic remitting/nonrelapsing
      experimental allergic encephalomyelitis.
PG  - 2262-6
AB  - Experimental allergic encephalomyelitis (EAE), the principal animal model of
      multiple sclerosis, is genetically controlled. To date, 13 disease-modifying loci
      have been identified in the mouse by whole genome scanning using an F2 intercross
      between EAE-susceptible SJL/J and EAE-resistant B10.S/DvTe mice. Two quantitative
      trait loci (QTL), eae6 and eae7, on chromosome 11 were identified by classical
      marker-specific linkage analysis and interval mapping. Both QTL were reported to 
      be associated with severity and duration of clinical signs. eae7 was subsequently
      shown to be a unique locus controlling the development of monophasic
      remitting/nonrelapsing EAE. In this study, composite interval mapping resolved
      eae6 into two linked QTL: eae6a at 0-13 cM is associated with disease severity,
      and eae6b at 19-28 cM associated with the duration of clinical signs.
      Additionally, composite interval mapping significantly refined the locations of
      eae6a, eae6b, and eae7, thereby facilitating systematic candidate gene screening 
      by cDNA sequencing of SJL/J and B10.S/DvTe alleles. Sequence polymorphisms were
      not seen in Lif and IL12 beta, candidate genes for eae6a and eae6b, respectively.
      Similarly, cDNA sequence polymorphisms in Nos2, Scya3, Scya4, Scya5, Scya6,
      Scya7, Scya9, Scya10, and Scya11 were excluded as candidates for eae7. However,
      multiple sequence polymorphisms resulting in significant amino acid substitutions
      were identified in Scya1 (TCA-3), Scya2 (monocyte chemoattractant protein
      (MCP)-1), and Scya12 (MCP-5). Given the role of chemokines in EAE, these sequence
      polymorphisms are promising candidates for eae7, a locus associated with severity
      of clinical signs and susceptibility to the shorter, less severe monophasic
      remitting/nonrelapsing form of disease.
FAU - Teuscher, C
AU  - Teuscher C
AD  - Department of Veterinary Pathobiology, University of Illinois at Urbana-Champaign
      61802, USA. cteusche@uiuc.edu
FAU - Butterfield, R J
AU  - Butterfield RJ
FAU - Ma, R Z
AU  - Ma RZ
FAU - Zachary, J F
AU  - Zachary JF
FAU - Doerge, R W
AU  - Doerge RW
FAU - Blankenhorn, E P
AU  - Blankenhorn EP
LA  - eng
SI  - GENBANK/AF065924
SI  - GENBANK/AF065925
SI  - GENBANK/AF065926
SI  - GENBANK/AF065927
SI  - GENBANK/AF065928
SI  - GENBANK/AF065929
SI  - GENBANK/AF065930
SI  - GENBANK/AF065931
SI  - GENBANK/AF065932
SI  - GENBANK/AF065933
SI  - GENBANK/AF065934
SI  - GENBANK/AF065935
SI  - GENBANK/AF065936
SI  - GENBANK/AF065937
SI  - GENBANK/AF065938
GR  - AI41747/AI/NIAID NIH HHS/United States
GR  - NS25519/NS/NINDS NIH HHS/United States
GR  - NS36526/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (CCL1 protein, human)
RN  - 0 (Ccl1 protein, mouse)
RN  - 0 (Ccl12 protein, mouse)
RN  - 0 (Chemokine CCL1)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CC)
RN  - 0 (Genetic Markers)
RN  - 0 (Monocyte Chemoattractant Proteins)
SB  - AIM
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chemokine CCL1
MH  - Chemokine CCL2/genetics
MH  - Chemokines/*genetics
MH  - *Chemokines, CC
MH  - Chromosomes, Human, Pair 11/immunology
MH  - Encephalomyelitis, Autoimmune, Experimental/*genetics/immunology
MH  - Genes, Overlapping/immunology
MH  - *Genetic Markers
MH  - Genetic Predisposition to Disease/genetics
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred Strains
MH  - Molecular Sequence Data
MH  - Monocyte Chemoattractant Proteins/genetics
MH  - Polymorphism, Genetic/*immunology
MH  - Quantitative Trait, Heritable
EDAT- 1999/08/10 00:00
MHDA- 1999/08/10 00:01
CRDT- 1999/08/10 00:00
PHST- 1999/08/10 00:00 [pubmed]
PHST- 1999/08/10 00:01 [medline]
PHST- 1999/08/10 00:00 [entrez]
AID - ji_v163n4p2262 [pii]
PST - ppublish
SO  - J Immunol. 1999 Aug 15;163(4):2262-6.