PMID- 10438587
OWN - NLM
STAT- MEDLINE
DCOM- 19990914
LR  - 20131121
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 251
IP  - 1
DP  - 1999 Aug 25
TI  - Nestin is expressed during development and in myotendinous and neuromuscular
      junctions in wild type and desmin knock-out mice.
PG  - 213-23
AB  - Desmin, the main component of intermediate filaments (IFs) in mature skeletal
      muscle, forms an interlinking scaffold around myofibrils with connections to the 
      sarcolemma and the nuclear membrane. Desmin is enriched in neuromuscular and
      myotendinous junctions. Mice lacking the desmin gene develop normally and
      reproduce. However, postnatally they develop a cardiomyopathy and a dystrophy in 
      highly used muscles. We have investigated whether and how neuromuscular and
      myotendinous junctions are affected and whether nestin compensates for the lack
      of desmin in the knock-out (K/O) mice. We show that neither neuromuscular nor
      myotendinous junctions were markedly affected in the desmin K/O mice. In
      neuromuscular junctions nestin was present between the postjunctional folds and
      the subneural nuclei and between the nucleus and the myofibrillar cytoskeleton.
      In myotendinous junctions nestin was present between myofibrils at the Z-disc
      level and in longitudinal strands close to and at the junction. Nestin expression
      at these specialized sites, as well as during myogenesis and myofibrillogenesis, 
      is independent of the presence of desmin. In desmin K/O mice nestin was also
      found in regenerating myofibers. The presence of nestin at neuromuscular and
      myotendinous junctions might provide enough strength for preservation and
      organization of the junctional areas, although desmin is lacking.
CI  - Copyright 1999 Academic Press.
FAU - Carlsson, L
AU  - Carlsson L
AD  - Department of Integrative Medical Biology, Section for Anatomy, Umea University, 
      Umea, SE-901 87, Sweden.
FAU - Li, Z
AU  - Li Z
FAU - Paulin, D
AU  - Paulin D
FAU - Thornell, L E
AU  - Thornell LE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Antibodies)
RN  - 0 (Connectin)
RN  - 0 (Desmin)
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nes protein, mouse)
RN  - 0 (Nestin)
SB  - IM
SB  - S
MH  - Aging
MH  - Animals
MH  - Animals, Newborn
MH  - Antibodies
MH  - Cell Differentiation
MH  - Connectin
MH  - Cytoplasm/metabolism
MH  - Desmin/genetics/*physiology
MH  - *Gene Expression Regulation, Developmental
MH  - Immunohistochemistry
MH  - Intermediate Filament Proteins/*metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Microscopy, Electron
MH  - Microscopy, Immunoelectron
MH  - *Muscle Development
MH  - Muscle Fibers, Skeletal/cytology/metabolism/physiology/ultrastructure
MH  - Muscle Proteins/metabolism
MH  - Muscle, Skeletal/cytology/embryology/*growth & development/ultrastructure
MH  - *Nerve Tissue Proteins
MH  - Nestin
MH  - Neuromuscular Junction/cytology/*metabolism/ultrastructure
MH  - Regeneration
MH  - Sarcolemma/metabolism/ultrastructure
MH  - Tendons/cytology/*metabolism/ultrastructure
EDAT- 1999/08/10 00:00
MHDA- 1999/08/10 00:01
CRDT- 1999/08/10 00:00
PHST- 1999/08/10 00:00 [pubmed]
PHST- 1999/08/10 00:01 [medline]
PHST- 1999/08/10 00:00 [entrez]
AID - 10.1006/excr.1999.4569 [doi]
AID - S0014-4827(99)94569-X [pii]
PST - ppublish
SO  - Exp Cell Res. 1999 Aug 25;251(1):213-23. doi: 10.1006/excr.1999.4569.