PMID- 10438528
OWN - NLM
STAT- MEDLINE
DCOM- 19990901
LR  - 20191217
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 33
DP  - 1999 Aug 13
TI  - hFOG-2, a novel zinc finger protein, binds the co-repressor mCtBP2 and modulates 
      GATA-mediated activation.
PG  - 23491-8
AB  - We have identified a novel human zinc finger protein, hFOG-2, which is related to
      but distinct from the murine transcription factor Friend-of-GATA-1 (mFOG-1). The 
      hFOG-2 gene was initially detected in K562 cells using a polymerase chain
      reaction approach with degenerate primers corresponding to zinc finger regions of
      mFOG-1. A murine homologue of hFOG-2 was also identified in the mouse expressed
      sequence tag data banks, indicating that a family of FOG genes exists in mammals.
      hFOG-2 appears to be widely expressed, while mFOG-1 is expressed primarily in
      erythroid and megakaryocytic cells and plays a fundamental role in the
      development of these lineages. Sequencing of the full-length hFOG-2 cDNA
      indicates that the interaction domains for transcription factors GATA-1 and
      mCtBP2 are both conserved and we have shown that this new FOG protein also
      physically interacts with these factors. We have demonstrated that hFOG-2, like
      mFOG-1, can act in concert with GATA-1 to activate gene expression from the p45
      NF-E2 promoter region, but that it can also act to repress GATA-mediated
      activation of additional reporter constructs. Finally, we have identified a
      repression domain in hFOG-2 and show that repression is dependent upon the
      integrity of the mCtBP2 interaction motif Pro-Ile-Asp-Leu-Ser.
FAU - Holmes, M
AU  - Holmes M
AD  - Centre for Thrombosis and Vascular Research, School of Pathology, University of
      New South Wales, Sydney, New South Wales 2052, Australia.
FAU - Turner, J
AU  - Turner J
FAU - Fox, A
AU  - Fox A
FAU - Chisholm, O
AU  - Chisholm O
FAU - Crossley, M
AU  - Crossley M
FAU - Chong, B
AU  - Chong B
LA  - eng
SI  - GENBANK/AF119334
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Erythroid-Specific DNA-Binding Factors)
RN  - 0 (GATA1 Transcription Factor)
RN  - 0 (GATA1 protein, human)
RN  - 0 (Gata1 protein, mouse)
RN  - 0 (NF-E2 Transcription Factor)
RN  - 0 (NF-E2 Transcription Factor, p45 Subunit)
RN  - 0 (NFE2 protein, human)
RN  - 0 (Nfe2 protein, mouse)
RN  - 0 (Phosphoproteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (ZFPM2 protein, human)
RN  - 0 (Zfpm2 protein, mouse)
RN  - EC 1.1.- (Ctbp2 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Erythroid-Specific DNA-Binding Factors
MH  - GATA1 Transcription Factor
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - NF-E2 Transcription Factor
MH  - NF-E2 Transcription Factor, p45 Subunit
MH  - Phosphoproteins/*metabolism
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Repressor Proteins/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Transcription Factors/*metabolism
MH  - *Zinc Fingers
EDAT- 1999/08/07 00:00
MHDA- 1999/08/07 00:01
CRDT- 1999/08/07 00:00
PHST- 1999/08/07 00:00 [pubmed]
PHST- 1999/08/07 00:01 [medline]
PHST- 1999/08/07 00:00 [entrez]
AID - 10.1074/jbc.274.33.23491 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 13;274(33):23491-8. doi: 10.1074/jbc.274.33.23491.