PMID- 10438527
OWN - NLM
STAT- MEDLINE
DCOM- 19990901
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 33
DP  - 1999 Aug 13
TI  - Isolation of mouse TFIID and functional characterization of TBP and TFIID in
      mediating estrogen receptor and chromatin transcription.
PG  - 23480-90
AB  - TFIID is a general transcription factor required for the assembly of the
      transcription machinery on most eukaryotic promoters transcribed by RNA
      polymerase II. Although the TATA-binding subunit (TBP) of TFIID is able to
      support core promoter and activator-dependent transcription under some
      circumstances, the roles of TBP-associated factors (TAF(II)s) in TFIID-mediated
      activation remain unclear. To define the evolutionarily conserved function of
      TFIID and to elucidate the roles of TAF(II)s in gene activation, we have cloned
      the mouse TAF(II)55 subunit of TFIID and further isolated mouse TFIID from a
      murine FM3A-derived cell line that constitutively expresses FLAG-tagged mouse
      TAF(II)55. Both mouse and human TFIIDs are capable of mediating transcriptional
      activation by Gal4 fusions containing different activation domains in a highly
      purified human cell-free transcription system devoid of TFIIA and Mediator.
      Although TAF(II)-independent activation by Gal4-VP16 can also be observed in this
      highly purified human transcription system with either mouse or yeast TBP,
      TAF(II)s are strictly required for estrogen receptor-mediated activation
      independently of the core promoter sequence. In addition, TAF(II)s are necessary 
      for transcription from a preassembled chromatin template. These findings clearly 
      demonstrate an essential role of TAF(II)s as a transcriptional coactivator for
      estrogen receptor and in chromatin transcription.
FAU - Wu, S Y
AU  - Wu SY
AD  - Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA.
FAU - Thomas, M C
AU  - Thomas MC
FAU - Hou, S Y
AU  - Hou SY
FAU - Likhite, V
AU  - Likhite V
FAU - Chiang, C M
AU  - Chiang CM
LA  - eng
SI  - GENBANK/AF144562
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chromatin)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (TATA-Box Binding Protein)
RN  - 0 (Transcription Factor TFIID)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcription Factors, TFII)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chromatin/*genetics
MH  - DNA Primers
MH  - DNA, Complementary
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - Receptors, Estrogen/*genetics
MH  - TATA-Box Binding Protein
MH  - Transcription Factor TFIID
MH  - Transcription Factors/genetics/*metabolism
MH  - Transcription Factors, TFII/*isolation & purification/metabolism
MH  - *Transcription, Genetic
EDAT- 1999/08/07 00:00
MHDA- 1999/08/07 00:01
CRDT- 1999/08/07 00:00
PHST- 1999/08/07 00:00 [pubmed]
PHST- 1999/08/07 00:01 [medline]
PHST- 1999/08/07 00:00 [entrez]
AID - 10.1074/jbc.274.33.23480 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 13;274(33):23480-90. doi: 10.1074/jbc.274.33.23480.