PMID- 10438493
OWN - NLM
STAT- MEDLINE
DCOM- 19990901
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 33
DP  - 1999 Aug 13
TI  - Localization of a new prostate-specific antigen-related serine protease gene,
      KLK4, is evidence for an expanded human kallikrein gene family cluster on
      chromosome 19q13.3-13.4.
PG  - 23210-4
AB  - The human tissue kallikrein (KLK) family of serine proteases, which is important 
      in post-translational processing events, currently consists of just three
      genes-tissue kallikrein (KLK1), KLK2, and prostate-specific antigen (PSA)
      (KLK3)-clustered at chromosome 19q13. 3-13.4. We identified an expressed sequence
      tag from an endometrial carcinoma cDNA library with 50% identity to the three
      known KLK genes. Primers designed to putative exon 2 and exon 3 regions from this
      novel kallikrein-related sequence were used to polymerase chain reaction-screen
      five cosmids spanning 130 kb around the KLK locus on chromosome 19. This new
      gene, which we have named KLK4, is 25 kb downstream of the KLK2 gene and follows 
      a region that includes two other putative KLK-like gene fragments. KLK4 spans 5.2
      kb, has an identical genomic structure-five exons and four introns-to the other
      KLK genes and is transcribed on the reverse strand, in the same direction as KLK1
      but opposite to that of KLK2 and KLK3. It encodes a 254-amino acid prepro-serine 
      protease that is most similar (78% identical) to pig enamel matrix serine
      protease but is also 37% identical to PSA. These data suggest that the human
      kallikrein gene family locus on chromosome 19 is larger than previously thought
      and also indicate a greater sequence divergence within this family compared with 
      the highly conserved rodent kallikrein genes.
FAU - Stephenson, S A
AU  - Stephenson SA
AD  - Centre for Molecular Biotechnology, School of Life Sciences, Queensland
      University of Technology, Brisbane, Australia 4001.
FAU - Verity, K
AU  - Verity K
FAU - Ashworth, L K
AU  - Ashworth LK
FAU - Clements, J A
AU  - Clements JA
LA  - eng
SI  - GENBANK/AF148532
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 9007-49-2 (DNA)
RN  - EC 3.4.21.- (Kallikreins)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (kallikrein 4)
RN  - EC 3.4.21.77 (Prostate-Specific Antigen)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - *Chromosomes, Human, Pair 19
MH  - DNA
MH  - Expressed Sequence Tags
MH  - Humans
MH  - Kallikreins/*genetics
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Prostate-Specific Antigen/*genetics
MH  - Serine Endopeptidases/*genetics
EDAT- 1999/08/07 00:00
MHDA- 1999/08/07 00:01
CRDT- 1999/08/07 00:00
PHST- 1999/08/07 00:00 [pubmed]
PHST- 1999/08/07 00:01 [medline]
PHST- 1999/08/07 00:00 [entrez]
AID - 10.1074/jbc.274.33.23210 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 13;274(33):23210-4. doi: 10.1074/jbc.274.33.23210.