PMID- 10438476
OWN - NLM
STAT- MEDLINE
DCOM- 19990901
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 33
DP  - 1999 Aug 13
TI  - Altered gene expression pattern in the fatty liver dystrophy mouse reveals
      impaired insulin-mediated cytoskeleton dynamics.
PG  - 23078-84
AB  - The mouse fatty liver dystrophy (fld) mutation is characterized by transient
      hypertriglyceridemia and fatty liver during the neonatal period, followed by
      development of a peripheral neuropathy. To uncover the metabolic pathway that is 
      disrupted by the fld mutation, we analyzed the altered pattern of gene expression
      in the fatty liver of fld neonates by representational difference analysis of
      cDNA. Differentially expressed genes detected include a novel member of the Ras
      superfamily of small GTP-binding proteins, a novel Ser/Thr kinase, and several
      actin cytoskeleton-associated proteins including actin, profilin, alpha-actinin, 
      and myosin light chain. Because these proteins have a potential functional link
      in the propagation of hormone signals, we investigated cytoskeleton dynamics in
      fld cells in response to hormone treatment. These studies revealed that
      preadipocytes from fld mice exhibit impaired formation of actin membrane ruffles 
      in response to insulin treatment. These findings suggest that the altered mRNA
      expression levels detected in fld tissue represent a compensatory response for
      the nonfunctional fld gene and that the fld gene product may be required for
      development of normal insulin response.
FAU - Klingenspor, M
AU  - Klingenspor M
AD  - Department of Medicine, University of California, and The Lipid Research
      Laboratory, West Los Angeles Veterans Affairs Medical Center, Los Angeles,
      California 90073, USA.
FAU - Xu, P
AU  - Xu P
FAU - Cohen, R D
AU  - Cohen RD
FAU - Welch, C
AU  - Welch C
FAU - Reue, K
AU  - Reue K
LA  - eng
SI  - GENBANK/AF054279
SI  - GENBANK/AF054280
SI  - GENBANK/AF054281
SI  - GENBANK/AF054282
SI  - GENBANK/AF054283
GR  - HL28481/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA Primers)
RN  - 0 (Insulin)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Cytoskeleton/*metabolism
MH  - DNA Primers
MH  - Expressed Sequence Tags
MH  - Fatty Liver/*genetics
MH  - *Gene Expression
MH  - Insulin/*metabolism
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Molecular Sequence Data
MH  - Proteins/chemistry/*genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction
EDAT- 1999/08/07 00:00
MHDA- 1999/08/07 00:01
CRDT- 1999/08/07 00:00
PHST- 1999/08/07 00:00 [pubmed]
PHST- 1999/08/07 00:01 [medline]
PHST- 1999/08/07 00:00 [entrez]
AID - 10.1074/jbc.274.33.23078 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 13;274(33):23078-84. doi: 10.1074/jbc.274.33.23078.