PMID- 10438470
OWN - NLM
STAT- MEDLINE
DCOM- 19990901
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 33
DP  - 1999 Aug 13
TI  - Histone acetyltransferase HBO1 interacts with the ORC1 subunit of the human
      initiator protein.
PG  - 23027-34
AB  - The origin recognition complex (ORC) is an initiator protein for DNA replication,
      but also effects transcriptional silencing in Saccharomyces cerevisiae and
      heterochromatin function in Drosophila. It is not known, however, whether any of 
      these functions of ORC is conserved in mammals. We report the identification of a
      novel protein, HBO1 (histone acetyltransferase binding to ORC), that interacts
      with human ORC1 protein, the largest subunit of ORC. HBO1 exists as part of a
      multisubunit complex that possesses histone H3 and H4 acetyltransferase
      activities. A fraction of the relatively abundant HBO1 protein associates with
      ORC1 in human cell extracts. HBO1 is a member of the MYST domain family that
      includes S. cerevisiae Sas2p, a protein involved in control of transcriptional
      silencing that also has been genetically linked to ORC function. Thus the
      interaction between ORC and a MYST domain acetyltransferase is widely conserved. 
      We suggest roles for ORC-mediated acetylation of chromatin in control of both DNA
      replication and gene expression.
FAU - Iizuka, M
AU  - Iizuka M
AD  - Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
FAU - Stillman, B
AU  - Stillman B
LA  - eng
SI  - GENBANK/AF074606
GR  - CA13106/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ORC1 protein, human)
RN  - 0 (Origin Recognition Complex)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (KAT7 protein, human)
RN  - EC 2.3.1.48 (KAT8 protein, human)
SB  - IM
MH  - Acetyltransferases/chemistry/genetics/*metabolism
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - DNA-Binding Proteins/*metabolism
MH  - HeLa Cells
MH  - Histone Acetyltransferases
MH  - Humans
MH  - Molecular Sequence Data
MH  - Origin Recognition Complex
MH  - Protein Binding
MH  - Saccharomyces cerevisiae/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic
EDAT- 1999/08/07 00:00
MHDA- 1999/08/07 00:01
CRDT- 1999/08/07 00:00
PHST- 1999/08/07 00:00 [pubmed]
PHST- 1999/08/07 00:01 [medline]
PHST- 1999/08/07 00:00 [entrez]
AID - 10.1074/jbc.274.33.23027 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 13;274(33):23027-34. doi: 10.1074/jbc.274.33.23027.