PMID- 10438455
OWN - NLM
STAT- MEDLINE
DCOM- 19990901
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 33
DP  - 1999 Aug 13
TI  - Human homolog of Caenorhabditis elegans sqv-3 gene is galactosyltransferase I
      involved in the biosynthesis of the glycosaminoglycan-protein linkage region of
      proteoglycans.
PG  - 22915-8
AB  - A cDNA encoding a novel galactosyltransferase was identified based on BLAST
      analysis of expressed sequence tags, and the cDNA clones were isolated from a
      human melanoma line library. The new cDNA sequence encoded a type II membrane
      protein with 327 amino acid sequence and showed 38% homology to the
      Caenorhabditis elegans sqv-3 gene involved in the vulval invagination and oocyte 
      development. Extracts from L cells transfected with the galactosyltransferase
      cDNA in an expression vector and a fusion protein with protein A exhibited marked
      galactosyltransferase activity specific for p-nitrophenyl-beta-D-xylopyranoside. 
      Moreover, transfection with the cloned cDNA restored glycosaminoglycan synthesis 
      of galactosyltransferase I-deficient Chinese hamster ovary mutant pgsB-761 cells.
      Analysis of the enzyme product by beta-galactosidase digestion, mass
      spectroscopy, and NMR spectroscopy revealed that the reaction product was formed 
      via beta-1,4 linkage, indicating that the enzyme is galactosyltransferase I
      (UDP-galactose:O-beta-D-xylosylprotein 4-beta-D-galactosyltransferase, EC
      2.4.1.133) involved in the synthesis of the glycosaminoglycan-protein linkage
      region of proteoglycans.
FAU - Okajima, T
AU  - Okajima T
AD  - Department of Biochemistry II, Nagoya University School of Medicine, Tsurumai,
      Nagoya 466-0065, Japan.
FAU - Yoshida, K
AU  - Yoshida K
FAU - Kondo, T
AU  - Kondo T
FAU - Furukawa, K
AU  - Furukawa K
LA  - eng
SI  - GENBANK/AB028600
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Helminth Proteins)
RN  - 0 (Proteoglycans)
RN  - EC 2.4.- (Glycosyltransferases)
RN  - EC 2.4.1.- (Galactosyltransferases)
RN  - EC 2.4.1.- (SQV-3 protein, C elegans)
RN  - EC 2.4.1.133 (xylosylprotein 4-beta-galactosyltransferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - Caenorhabditis elegans/*genetics
MH  - *Caenorhabditis elegans Proteins
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Complementary
MH  - Galactosyltransferases/chemistry/*genetics/metabolism
MH  - Glycosaminoglycans/*metabolism
MH  - Glycosyltransferases/*genetics/metabolism
MH  - Helminth Proteins/*genetics/metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Proteoglycans/*biosynthesis/chemistry
MH  - Sequence Homology, Amino Acid
EDAT- 1999/08/07 00:00
MHDA- 1999/08/07 00:01
CRDT- 1999/08/07 00:00
PHST- 1999/08/07 00:00 [pubmed]
PHST- 1999/08/07 00:01 [medline]
PHST- 1999/08/07 00:00 [entrez]
AID - 10.1074/jbc.274.33.22915 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Aug 13;274(33):22915-8. doi: 10.1074/jbc.274.33.22915.