PMID- 10437795
OWN - NLM
STAT- MEDLINE
DCOM- 19990816
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 455
IP  - 3
DP  - 1999 Jul 23
TI  - A complex containing betaTrCP recruits Cdc34 to catalyse ubiquitination of
      IkappaBalpha.
PG  - 311-4
AB  - Activation of transcription factor NF-kappaB is accomplished by degradation of
      its inhibitor IkappaBalpha. Signal induced phosphorylation of IkappaBalpha on
      serine 32 and 36 targets the protein for ubiquitination on lysine 21 and 22. Here
      we use a phosphorylated peptide substrate representing residues 20-43 of
      IkappaBalpha to investigate requirements for ubiquitination of IkappaBalpha.
      Phosphorylation dependent polyubiquitination is carried out by a multiprotein
      complex containing betaTrCP, Skp1 and Cdc53 (Cull). In the presence of ubiquitin 
      activating enzyme and the protein complex containing betaTrCP, polyubiquitination
      of IkappaBalpha peptide was dependent on the presence of Cdc34, while Ubc5 only
      stimulated mono- and di-ubiquitination.
FAU - Vuillard, L
AU  - Vuillard L
AD  - School of Biomedical Science, University of St. Andrews, Fife, UK.
FAU - Nicholson, J
AU  - Nicholson J
FAU - Hay, R T
AU  - Hay RT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (BTRC protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (NF-kappa B)
RN  - 0 (NFKBIA protein, human)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Ubiquitins)
RN  - 0 (beta-Transducin Repeat-Containing Proteins)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - EC 2.3.2.23 (CDC34 protein, human)
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
RN  - EC 2.3.2.23 (Ubiquitin-Protein Ligase Complexes)
RN  - EC 2.3.2.27 (Anaphase-Promoting Complex-Cyclosome)
RN  - EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 6.- (Ligases)
RN  - EC 6.3.2.- (Peptide Synthases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Anaphase-Promoting Complex-Cyclosome
MH  - Animals
MH  - COS Cells
MH  - DNA-Binding Proteins/chemistry/genetics/*metabolism
MH  - GTP-Binding Proteins/chemistry/genetics/*metabolism
MH  - Humans
MH  - *I-kappa B Proteins
MH  - Ligases/*metabolism
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - Multiprotein Complexes
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/metabolism
MH  - Peptide Fragments/chemistry/genetics/metabolism
MH  - Peptide Synthases/chemistry/metabolism
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - SKP Cullin F-Box Protein Ligases
MH  - Ubiquitin-Conjugating Enzymes
MH  - *Ubiquitin-Protein Ligase Complexes
MH  - Ubiquitin-Protein Ligases
MH  - Ubiquitins/metabolism
MH  - beta-Transducin Repeat-Containing Proteins
EDAT- 1999/08/07 00:00
MHDA- 1999/08/07 00:01
CRDT- 1999/08/07 00:00
PHST- 1999/08/07 00:00 [pubmed]
PHST- 1999/08/07 00:01 [medline]
PHST- 1999/08/07 00:00 [entrez]
AID - S0014579399008959 [pii]
AID - 10.1016/s0014-5793(99)00895-9 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Jul 23;455(3):311-4. doi: 10.1016/s0014-5793(99)00895-9.