PMID- 10436421
OWN - NLM
STAT- MEDLINE
DCOM- 19990817
LR  - 20180213
IS  - 1010-4283 (Print)
IS  - 1010-4283 (Linking)
VI  - 20
IP  - 5
DP  - 1999 Sep-Oct
TI  - Four carcinoembryonic antigen subfamily members, CEA, NCA, BGP and CGM2,
      selectively expressed in the normal human colonic epithelium, are integral
      components of the fuzzy coat.
PG  - 277-92
AB  - To elucidate which of the seven transcriptionally active genes of the
      carcinoembryonic antigen (CEA) subfamily are expressed in human colon, we first
      examined mRNA expression using reverse transcriptase PCR. The result showed the
      CEA, nonspecific crossreacting antigen 50/90 (NCA), biliary glycoprotein (BGP),
      and carcinoembryonic antigen gene family member 2 (CGM2) mRNAs were expressed in 
      the colon. To determine the cellular sources of these members within normal
      colonic mucosa, in situ hybridization and immunocytochemistry were then
      performed. CEA and NCA mRNAs were clearly detectable in the cytoplasm of columnar
      and goblet cells at the free luminal surface and the upper crypts with low
      hybridization in the mid crypt and the crypt base. In contrast, BGP and CGM2
      mRNAs were restricted only to columnar cells at the upper third of the crypts and
      the luminal surface. Colon epithelium expression of CEA, NCA, BGP and CGM2
      coincided with that of corresponding mRNAs. Ultrastructurally, CEA, NCA, BGP and 
      CGM2 were localized mainly to the apical surface glycocalyx, the fuzzy coat, of
      columnar cells. Interestingly, these molecules were localized in different
      microdomains within the fuzzy coat. Furthermore, BGP was highly expressed in the 
      fuzzy coat of cryptal caveolated cells. As integral components of the fuzzy coat,
      CEA, NCA, BGP and CGM2 can hardly function as intercellular adhesion molecules;
      they possibly play an important role in epithelial-microbial interactions.
FAU - Frangsmyr, L
AU  - Frangsmyr L
AD  - Department of Immunology, Umea University, Umea, Sweden.
FAU - Baranov, V
AU  - Baranov V
FAU - Hammarstrom, S
AU  - Hammarstrom S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (CD66 antigens)
RN  - 0 (CEACAM7 protein, human)
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Antigens, CD
MH  - Antigens, Neoplasm/biosynthesis
MH  - Carcinoembryonic Antigen/*biosynthesis
MH  - Cell Adhesion Molecules/*biosynthesis
MH  - Colon/*metabolism
MH  - GPI-Linked Proteins
MH  - Glycocalyx/*metabolism
MH  - Glycoproteins/*biosynthesis
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Intestinal Mucosa/*metabolism
MH  - Membrane Glycoproteins/*biosynthesis
MH  - Microscopy, Immunoelectron
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 1999/08/07 00:00
MHDA- 1999/08/07 00:01
CRDT- 1999/08/07 00:00
PHST- 1999/08/07 00:00 [pubmed]
PHST- 1999/08/07 00:01 [medline]
PHST- 1999/08/07 00:00 [entrez]
AID - 30075 [pii]
AID - 10.1159/000030075 [doi]
PST - ppublish
SO  - Tumour Biol. 1999 Sep-Oct;20(5):277-92. doi: 10.1159/000030075.