PMID- 10436176
OWN - NLM
STAT- MEDLINE
DCOM- 19990914
LR  - 20190831
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 49
IP  - 10
DP  - 1999 Sep
TI  - The complete primary structure of mouse 20S proteasomes.
PG  - 835-42
AB  - The proteasome is a large multicatalytic proteinase that plays a role in the
      generation of peptides for presentation by major histocompatibility complex class
      I molecules. The 20S proteolytic core of mammalian proteasomes is assembled from 
      a group of 17 protein subunits that generate a distinctive pattern of spots upon 
      two-dimensional gel electrophoresis. The genes for most of these subunits have
      been cloned from humans and rats. We isolated cDNA clones for the mouse
      orthologues of ten of the subunits [PSMA1 (C2), PSMA2 (C3), PSMA3 (C8), PSMA4
      (C9), PSMA5 (ZETA), PSMA6 (IOTA), PSMA7 (C6-I), PSMB2 (C7-I), PSMB3 (C10-II), and
      PSMB5 (X)] to complete the cloning of all of the mouse subunits. Using antisera
      raised against these subunits or their orthologues, we verified the identity of
      these proteins by two-dimensional NEPHGE-PAGE.
FAU - Elenich, L A
AU  - Elenich LA
AD  - Howard Hughes Medical Institute, and Departments of Molecular Genetics and Cell
      Biology, University of Cincinnati, 231 Bethesda Avenue, Cincinnati, OH
      45267-0524, USA.
FAU - Nandi, D
AU  - Nandi D
FAU - Kent, A E
AU  - Kent AE
FAU - McCluskey, T S
AU  - McCluskey TS
FAU - Cruz, M
AU  - Cruz M
FAU - Iyer, M N
AU  - Iyer MN
FAU - Woodward, E C
AU  - Woodward EC
FAU - Conn, C W
AU  - Conn CW
FAU - Ochoa, A L
AU  - Ochoa AL
FAU - Ginsburg, D B
AU  - Ginsburg DB
FAU - Monaco, J J
AU  - Monaco JJ
LA  - eng
SI  - GENBANK/AF019661
SI  - GENBANK/AF019662
SI  - GENBANK/AF060087
SI  - GENBANK/AF060088
SI  - GENBANK/AF060089
SI  - GENBANK/AF060090
SI  - GENBANK/AF060091
SI  - GENBANK/AF060092
SI  - GENBANK/AF060093
SI  - GENBANK/AF099733
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Multienzyme Complexes)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
RN  - EC 3.4.25.1 (Psmb5 protein, mouse)
RN  - EC 3.4.25.1 (Psmb5 protein, rat)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Cysteine Endopeptidases/chemistry/*genetics/isolation & purification
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Multienzyme Complexes/chemistry/*genetics/isolation & purification
MH  - Phylogeny
MH  - Proteasome Endopeptidase Complex
MH  - Protein Conformation
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
EDAT- 1999/08/07 00:00
MHDA- 1999/08/07 00:01
CRDT- 1999/08/07 00:00
PHST- 1999/08/07 00:00 [pubmed]
PHST- 1999/08/07 00:01 [medline]
PHST- 1999/08/07 00:00 [entrez]
AID - 90490835.251 [pii]
AID - 10.1007/s002510050562 [doi]
PST - ppublish
SO  - Immunogenetics. 1999 Sep;49(10):835-42. doi: 10.1007/s002510050562.