PMID- 10436156
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 285
IP  - 5429
DP  - 1999 Aug 6
TI  - Requirement of Rsk-2 for epidermal growth factor-activated phosphorylation of
      histone H3.
PG  - 886-91
AB  - During the immediate-early response of mammalian cells to mitogens, histone H3 is
      rapidly and transiently phosphorylated by one or more unidentified kinases.
      Rsk-2, a member of the pp90rsk family of kinases implicated in growth control,
      was required for epidermal growth factor (EGF)-stimulated phosphorylation of H3. 
      RSK-2 mutations in humans are linked to Coffin-Lowry syndrome (CLS). Fibroblasts 
      derived from a CLS patient failed to exhibit EGF-stimulated phosphorylation of
      H3, although H3 was phosphorylated during mitosis. Introduction of the wild-type 
      RSK-2 gene restored EGF-stimulated phosphorylation of H3 in CLS cells. In
      addition, disruption of the RSK-2 gene by homologous recombination in murine
      embryonic stem cells abolished EGF-stimulated phosphorylation of H3. H3 appears
      to be a direct or indirect target of Rsk-2, suggesting that chromatin remodeling 
      might contribute to mitogen-activated protein kinase-regulated gene expression.
FAU - Sassone-Corsi, P
AU  - Sassone-Corsi P
AD  - Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS, INSERM,
      ULP, B. P. 163, 67404 Illkirch-Strasbourg, France. paolosc@igbmc.u-strasbg.fr
FAU - Mizzen, C A
AU  - Mizzen CA
FAU - Cheung, P
AU  - Cheung P
FAU - Crosio, C
AU  - Crosio C
FAU - Monaco, L
AU  - Monaco L
FAU - Jacquot, S
AU  - Jacquot S
FAU - Hanauer, A
AU  - Hanauer A
FAU - Allis, C D
AU  - Allis CD
LA  - eng
GR  - GM40922/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Histones)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - 3T3 Cells
MH  - Abnormalities, Multiple/genetics/metabolism
MH  - Animals
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Cell Line, Transformed
MH  - Cell Nucleus/metabolism
MH  - Cells, Cultured
MH  - Epidermal Growth Factor/*pharmacology
MH  - Gene Expression Regulation
MH  - Gene Targeting
MH  - Histones/*metabolism
MH  - Humans
MH  - Mice
MH  - Mitosis
MH  - Mutation
MH  - Phosphorylation
MH  - Ribosomal Protein S6 Kinases/genetics/*metabolism
MH  - Signal Transduction
MH  - Stem Cells/cytology/metabolism
MH  - Syndrome
EDAT- 1999/08/07 00:00
MHDA- 1999/08/07 00:01
CRDT- 1999/08/07 00:00
PHST- 1999/08/07 00:00 [pubmed]
PHST- 1999/08/07 00:01 [medline]
PHST- 1999/08/07 00:00 [entrez]
AID - 7736 [pii]
AID - 10.1126/science.285.5429.886 [doi]
PST - ppublish
SO  - Science. 1999 Aug 6;285(5429):886-91. doi: 10.1126/science.285.5429.886.