PMID- 10436054
OWN - NLM
STAT- MEDLINE
DCOM- 19990901
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 16
DP  - 1999 Aug 15
TI  - Thyroid hormone regulates reelin and dab1 expression during brain development.
PG  - 6979-93
AB  - The reelin and dab1 genes are necessary for appropriate neuronal migration and
      lamination during brain development. Since these processes are controlled by
      thyroid hormone, we studied the effect of thyroid hormone deprivation and
      administration on the expression of reelin and dab1. As shown by Northern
      analysis, in situ hybridization, and immunohistochemistry studies, hypothyroid
      rats expressed decreased levels of reelin RNA and protein during the perinatal
      period [embryonic day 18 (E18) and postnatal day 0 (P0)]. The effect was evident 
      in Cajal-Retzius cells of cortex layer I, as well as in layers V/VI, hippocampus,
      and granular neurons of the cerebellum. At later ages, however, Reelin was more
      abundant in the cortex, hippocampus, cerebellum, and olfactory bulb of
      hypothyroid rats (P5), and no differences were detected at P15. Conversely, Dab1 
      levels were higher at P0, and lower at P5 in hypothyroid animals. In line with
      these results, reelin RNA and protein levels were higher in cultured hippocampal 
      slices from P0 control rats compared to those from hypothyroid animals.
      Significantly, thyroid-dependent regulation of reelin and dab1 was confirmed in
      vivo and in vitro by hormone treatment of hypothyroid rats and organotypic
      cultures, respectively. In both cases, thyroid hormone led to an increase in
      reelin expression. Our data suggest that the effects of thyroid hormone on
      neuronal migration may be in part mediated through the control of reelin and dab1
      expression during brain ontogenesis.
FAU - Alvarez-Dolado, M
AU  - Alvarez-Dolado M
AD  - Instituto de Investigaciones Biomedicas "Alberto Sols", Consejo Superior de
      Investigaciones Cientificas, Universidad Autonoma de Madrid, 28029 Madrid, Spain.
FAU - Ruiz, M
AU  - Ruiz M
FAU - Del Rio, J A
AU  - Del Rio JA
FAU - Alcantara, S
AU  - Alcantara S
FAU - Burgaya, F
AU  - Burgaya F
FAU - Sheldon, M
AU  - Sheldon M
FAU - Nakajima, K
AU  - Nakajima K
FAU - Bernal, J
AU  - Bernal J
FAU - Howell, B W
AU  - Howell BW
FAU - Curran, T
AU  - Curran T
FAU - Soriano, E
AU  - Soriano E
FAU - Munoz, A
AU  - Munoz A
LA  - eng
GR  - CA41702/CA/NCI NIH HHS/United States
GR  - P30 CA21765/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for
      Neuroscience
JID - 8102140
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (DAB1 protein, Neurospora crassa)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Thyroid Hormones)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (reelin protein)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain/embryology/growth & development/*metabolism
MH  - Cell Adhesion Molecules, Neuronal/*genetics
MH  - Cell Movement/physiology
MH  - Cerebral Cortex/metabolism
MH  - Down-Regulation
MH  - Embryonic and Fetal Development/physiology
MH  - Extracellular Matrix Proteins/*genetics
MH  - Fungal Proteins/*genetics
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Hypothyroidism/physiopathology
MH  - In Vitro Techniques
MH  - Nerve Tissue Proteins/*genetics
MH  - Neurons/cytology
MH  - Rats
MH  - Rats, Wistar
MH  - Serine Endopeptidases
MH  - Thyroid Hormones/*physiology
PMC - PMC6782883
EDAT- 1999/08/06 00:00
MHDA- 1999/08/06 00:01
CRDT- 1999/08/06 00:00
PHST- 1999/08/06 00:00 [pubmed]
PHST- 1999/08/06 00:01 [medline]
PHST- 1999/08/06 00:00 [entrez]
PST - ppublish
SO  - J Neurosci. 1999 Aug 15;19(16):6979-93.