PMID- 10436050
OWN - NLM
STAT- MEDLINE
DCOM- 19990901
LR  - 20191210
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 16
DP  - 1999 Aug 15
TI  - Characterization of the glutamate receptor-interacting proteins GRIP1 and GRIP2.
PG  - 6930-41
AB  - The molecular mechanisms underlying the targeting and localization of glutamate
      receptors at postsynaptic sites is poorly understood. Recently, we have
      identified a PDZ domain-containing protein, glutamate receptor-interacting
      protein 1 (GRIP1), which specifically binds to the C termini of AMPA receptor
      subunits and may be involved in the synaptic targeting of these receptors. Here, 
      we report the cloning of GRIP2, a homolog of GRIP1, and the characterization of
      the GRIP1 and GRIP2 proteins in the rat CNS. GRIP1 and GRIP2 are approximately
      130 kDa proteins that are highly enriched in brain. GRIP1 and GRIP2 are widely
      expressed in brain, with the highest levels found in the cerebral cortex,
      hippocampus, and olfactory bulb. Biochemical studies show that GRIP1 and GRIP2
      are enriched in synaptic plasma membrane and postsynaptic density fractions.
      GRIP1 is expressed early in embryonic development before the expression of AMPA
      receptors and peaks in expression at postnatal day 8-10. In contrast, GRIP2 is
      expressed relatively late in development and parallels the expression of AMPA
      receptors. Immunohistochemistry using the GRIP1 antibodies demonstrated that
      GRIP1 is expressed in neurons in a somatodendritic staining pattern. At the
      ultrastructural level, DAB and immunogold electromicroscopy studies showed that
      GRIP1 was enriched in dendritic spines near the postsynaptic density and was
      expressed in dendritic shafts and in peri-Golgi regions in the neuronal soma.
      GRIP1 appeared to be clustered at both glutamatergic and GABAergic synapses.
      These results suggest that GRIP1 and GRIP2 are AMPA receptor binding proteins
      potentially involved in the targeting of AMPA receptors to synapses. GRIP1 also
      may play functional roles at both excitatory and inhibitory synapses, as well as 
      in early neuronal development.
FAU - Dong, H
AU  - Dong H
AD  - Howard Hughes Medical Institute, Department of Neuroscience, The Johns Hopkins
      University School of Medicine, Baltimore, Maryland 21205, USA.
FAU - Zhang, P
AU  - Zhang P
FAU - Song, I
AU  - Song I
FAU - Petralia, R S
AU  - Petralia RS
FAU - Liao, D
AU  - Liao D
FAU - Huganir, R L
AU  - Huganir RL
LA  - eng
SI  - GENBANK/AF205193
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for
      Neuroscience
JID - 8102140
RN  - 0 (Carrier Proteins)
RN  - 0 (Grip2 protein, mouse)
RN  - 0 (Grip2 protein, rat)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Ncoa2 protein, mouse)
RN  - 0 (Ncoa2 protein, rat)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nuclear Receptor Coactivator 2)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Specificity
MH  - Brain Chemistry/physiology
MH  - Carrier Proteins/genetics/*isolation & purification
MH  - Cells, Cultured
MH  - Cerebral Cortex/chemistry
MH  - Cloning, Molecular
MH  - Hippocampus/chemistry
MH  - Immunoenzyme Techniques
MH  - Immunohistochemistry
MH  - Intercellular Signaling Peptides and Proteins
MH  - Intracellular Signaling Peptides and Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/genetics/*isolation & purification
MH  - Nuclear Receptor Coactivator 2
MH  - Rats
MH  - Receptors, AMPA/metabolism
MH  - Transcription Factors/genetics/*isolation & purification
PMC - PMC6782851
EDAT- 1999/08/06 00:00
MHDA- 1999/08/06 00:01
CRDT- 1999/08/06 00:00
PHST- 1999/08/06 00:00 [pubmed]
PHST- 1999/08/06 00:01 [medline]
PHST- 1999/08/06 00:00 [entrez]
PST - ppublish
SO  - J Neurosci. 1999 Aug 15;19(16):6930-41.