PMID- 10436028
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 10
IP  - 8
DP  - 1999 Aug
TI  - Characterization of a fourth adaptor-related protein complex.
PG  - 2787-802
AB  - Adaptor protein complexes (APs) function as vesicle coat components in different 
      membrane traffic pathways; however, there are a number of pathways for which
      there is still no candidate coat. To find novel coat components related to AP
      complexes, we have searched the expressed sequence tag database and have
      identified, cloned, and sequenced a new member of each of the four AP subunit
      families. We have shown by a combination of coimmunoprecipitation and yeast
      two-hybrid analysis that these four proteins (epsilon, beta4, mu4, and sigma4)
      are components of a novel adaptor-like heterotetrameric complex, which we are
      calling AP-4. Immunofluorescence reveals that AP-4 is localized to approximately 
      10-20 discrete dots in the perinuclear region of the cell. This pattern is
      disrupted by treating the cells with brefeldin A, indicating that, like other
      coat proteins, the association of AP-4 with membranes is regulated by the small
      GTPase ARF. Immunogold electron microscopy indicates that AP-4 is associated with
      nonclathrin-coated vesicles in the region of the trans-Golgi network. The mu4
      subunit of the complex specifically interacts with a tyrosine-based sorting
      signal, indicating that, like the other three AP complexes, AP-4 is involved in
      the recognition and sorting of cargo proteins with tyrosine-based motifs. AP-4 is
      of relatively low abundance, but it is expressed ubiquitously, suggesting that it
      participates in a specialized trafficking pathway but one that is required in all
      cell types.
FAU - Hirst, J
AU  - Hirst J
AD  - University of Cambridge, Department of Clinical Biochemistry and Cambridge
      Institute for Medical Research, Cambridge CB2 2XY, England.
FAU - Bright, N A
AU  - Bright NA
FAU - Rous, B
AU  - Rous B
FAU - Robinson, M S
AU  - Robinson MS
LA  - eng
SI  - GENBANK/AF155156
SI  - GENBANK/AF155157
SI  - GENBANK/AF155158
SI  - GENBANK/AF155159
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 42HK56048U (Tyrosine)
SB  - IM
MH  - Adaptor Proteins, Vesicular Transport
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Humans
MH  - Microscopy, Electron
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*genetics/*metabolism
MH  - Phosphoproteins/*genetics/*metabolism
MH  - Proteins/*genetics/*metabolism
MH  - Rats
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction
MH  - Tyrosine/metabolism
PMC - PMC25515
EDAT- 1999/08/06 00:00
MHDA- 1999/08/06 00:01
CRDT- 1999/08/06 00:00
PHST- 1999/08/06 00:00 [pubmed]
PHST- 1999/08/06 00:01 [medline]
PHST- 1999/08/06 00:00 [entrez]
AID - 10.1091/mbc.10.8.2787 [doi]
PST - ppublish
SO  - Mol Biol Cell. 1999 Aug;10(8):2787-802. doi: 10.1091/mbc.10.8.2787.