PMID- 10436018
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 10
IP  - 8
DP  - 1999 Aug
TI  - The Cdc6 nucleotide-binding site regulates its activity in DNA replication in
      human cells.
PG  - 2631-45
AB  - The Cdc6 protein of budding yeast and its homologues in other species play an
      essential role in the initiation of DNA replication. A cDNA encoding a human
      homologue of Cdc6 (HsCdc6) has been cloned and expressed as a fusion protein in a
      soluble and functionally active form. The purified protein bound specifically to 
      ATP and slowly hydrolyzed it, whereas HsCdc6 mutants containing amino acid
      substitutions in the Walker A or B motifs were defective. The mutant proteins
      retained the ability to bind HsOrc1 and HsCdc6 but displayed aberrant
      conformations in the presence of nucleotides. Microinjection of either mutant
      protein into human cells in G1 inhibited DNA replication, suggesting that ATP
      binding and hydrolysis by HsCdc6 are essential for DNA replication.
FAU - Herbig, U
AU  - Herbig U
AD  - Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee
      37235, USA.
FAU - Marlar, C A
AU  - Marlar CA
FAU - Fanning, E
AU  - Fanning E
LA  - eng
SI  - GENBANK/U77949
GR  - R01 GM052948/GM/NIGMS NIH HHS/United States
GR  - GM-52948/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (CDC6 protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleotides)
RN  - 0 (Origin Recognition Complex)
RN  - 0 (Recombinant Proteins)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 5.99.1.3 (DNA Topoisomerases, Type II)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Baculoviridae/genetics
MH  - Binding Sites
MH  - Cell Cycle Proteins/genetics/isolation & purification/*metabolism
MH  - *DNA Replication
MH  - DNA Topoisomerases, Type II/chemistry/genetics/metabolism
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - G1 Phase/genetics
MH  - HeLa Cells/metabolism
MH  - Humans
MH  - Hydrolysis
MH  - Kinetics
MH  - Microinjections
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Nuclear Proteins/genetics/isolation & purification/*metabolism
MH  - Nucleotides/*metabolism
MH  - Origin Recognition Complex
MH  - Protein Conformation
MH  - Recombinant Proteins/genetics/isolation & purification/metabolism
MH  - S Phase/genetics
PMC - PMC25495
EDAT- 1999/08/06 00:00
MHDA- 1999/08/06 00:01
CRDT- 1999/08/06 00:00
PHST- 1999/08/06 00:00 [pubmed]
PHST- 1999/08/06 00:01 [medline]
PHST- 1999/08/06 00:00 [entrez]
AID - 10.1091/mbc.10.8.2631 [doi]
PST - ppublish
SO  - Mol Biol Cell. 1999 Aug;10(8):2631-45. doi: 10.1091/mbc.10.8.2631.