PMID- 10435631
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20171116
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 29
DP  - 1999 Jul 22
TI  - The zinc finger protein A20 interacts with a novel anti-apoptotic protein which
      is cleaved by specific caspases.
PG  - 4182-90
AB  - A20 is a Cys2/Cys2 zinc finger protein which is induced by a variety of
      inflammatory stimuli and which has been characterized as an inhibitor of cell
      death by a yet unknown mechanism. In order to clarify its molecular mechanism of 
      action, we used the yeast two-hybrid system to screen for proteins that interact 
      with A20. A cDNA fragment was isolated which encoded a portion of a novel protein
      (TXBP151), which was recently found to be a human T-cell leukemia virus type-I
      (HTLV-I) Tax-binding protein. The full-length 2386 bp TXBP151 mRNA encodes a
      protein of 86 kDa. Like A20, overexpression of TXBP151 could inhibit apoptosis
      induced by tumour necrosis factor (TNF) in NIH3T3 cells. Moreover, transfection
      of antisense TXBP151 partially abolished the anti-apoptotic effect of A20.
      Furthermore, apoptosis induced by TNF or CD95 (Fas/APO-1) was associated with
      proteolysis of TXBP151. This degradation could be inhibited by the broad-spectrum
      caspase inhibitor zVAD-fmk or by expression of the cowpox virus-derived inhibitor
      CrmA, suggesting that TXBP151 is a novel substrate for caspase family members.
      TXBP151 was indeed found to be specifically cleaved in vitro by members of the
      caspase-3-like subfamily, viz. caspase-3, caspase-6 and caspase-7. Thus TXBP151
      appears to be a novel A20-binding protein which might mediate the anti-apoptotic 
      activity of A20, and which can be processed by specific caspases.
FAU - De Valck, D
AU  - De Valck D
AD  - Department of Molecular Biology, Flanders Interuniversity Institute for
      Biotechnology and University of Gent, Belgium.
FAU - Jin, D Y
AU  - Jin DY
FAU - Heyninck, K
AU  - Heyninck K
FAU - Van de Craen, M
AU  - Van de Craen M
FAU - Contreras, R
AU  - Contreras R
FAU - Fiers, W
AU  - Fiers W
FAU - Jeang, K T
AU  - Jeang KT
FAU - Beyaert, R
AU  - Beyaert R
LA  - eng
SI  - GENBANK/U33821
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Amino Acid Chloromethyl Ketones)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Serpins)
RN  - 0 (TAX1BP1 protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Viral Proteins)
RN  - 0 (benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone)
RN  - 0 (fas Receptor)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 96282-35-8 (interleukin-1beta-converting enzyme inhibitor)
RN  - EC 3.4.19.12 (TNFAIP3 protein, human)
RN  - EC 3.4.19.12 (Tumor Necrosis Factor alpha-Induced Protein 3)
RN  - EC 3.4.22.- (Caspases)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.- (Tnfaip3 protein, mouse)
SB  - IM
SB  - X
MH  - 3T3 Cells
MH  - Amino Acid Chloromethyl Ketones/pharmacology
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Apoptosis/drug effects
MH  - Base Sequence
MH  - Carrier Proteins/isolation & purification/*metabolism
MH  - Caspases/*metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cysteine Endopeptidases
MH  - Cysteine Proteinase Inhibitors/pharmacology
MH  - DNA, Complementary/genetics
MH  - DNA-Binding Proteins
MH  - Dactinomycin/pharmacology
MH  - Genes
MH  - HeLa Cells
MH  - Humans
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Neoplasm Proteins
MH  - Nuclear Proteins
MH  - Nucleic Acid Synthesis Inhibitors/pharmacology
MH  - Oligonucleotides, Antisense/pharmacology
MH  - Protein Binding
MH  - *Protein Processing, Post-Translational
MH  - Proteins/*metabolism
MH  - RNA, Messenger/genetics
MH  - Recombinant Fusion Proteins/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - Serpins/physiology
MH  - Substrate Specificity
MH  - Transfection
MH  - Tumor Necrosis Factor alpha-Induced Protein 3
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - *Viral Proteins
MH  - *Zinc Fingers
MH  - fas Receptor/physiology
EDAT- 1999/08/06 00:00
MHDA- 1999/08/06 00:01
CRDT- 1999/08/06 00:00
PHST- 1999/08/06 00:00 [pubmed]
PHST- 1999/08/06 00:01 [medline]
PHST- 1999/08/06 00:00 [entrez]
AID - 10.1038/sj.onc.1202787 [doi]
PST - ppublish
SO  - Oncogene. 1999 Jul 22;18(29):4182-90. doi: 10.1038/sj.onc.1202787.