PMID- 10435581
OWN - NLM
STAT- MEDLINE
DCOM- 19990811
LR  - 20181201
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 11
IP  - 1
DP  - 1999 Jul
TI  - A novel, high endothelial venule-specific sulfotransferase expresses 6-sulfo
      sialyl Lewis(x), an L-selectin ligand displayed by CD34.
PG  - 79-89
AB  - L-selectin mediates lymphocyte homing by facilitating lymphocyte adhesion to
      unique carbohydrate ligands, sulfated sialyl Lewis(x), which are expressed on
      high endothelial venules (HEV) in secondary lymphoid organs. The nature of the
      sulfotransferase(s) that contribute to sulfation of such L-selectin
      counterreceptors has been uncertain. We herein describe a novel L-selectin ligand
      sulfotransferase, termed LSST, that directs the synthesis of the 6-sulfo sialyl
      Lewis(x) on L-selectin counterreceptors CD34, GlyCAM-1, and MAdCAM-1. LSST is
      predominantly expressed in HEV and exhibits striking catalytic preference for
      core 2-branched mucin-type O-glycans as found in natural L-selectin
      counterreceptors. LSST enhances L-selectin-mediated adhesion under shear compared
      to nonsulfated controls. LSST therefore corresponds to an HEV-specific
      sulfotransferase that contributes to the biosynthesis of L-selectin ligands
      required for lymphocyte homing.
FAU - Hiraoka, N
AU  - Hiraoka N
AD  - Glycobiology Program, Cancer Research Center, The Burnham Institute, La Jolla,
      California 92037, USA.
FAU - Petryniak, B
AU  - Petryniak B
FAU - Nakayama, J
AU  - Nakayama J
FAU - Tsuboi, S
AU  - Tsuboi S
FAU - Suzuki, M
AU  - Suzuki M
FAU - Yeh, J C
AU  - Yeh JC
FAU - Izawa, D
AU  - Izawa D
FAU - Tanaka, T
AU  - Tanaka T
FAU - Miyasaka, M
AU  - Miyasaka M
FAU - Lowe, J B
AU  - Lowe JB
FAU - Fukuda, M
AU  - Fukuda M
LA  - eng
SI  - GENBANK/AF109155
GR  - P01AI33189/AI/NIAID NIH HHS/United States
GR  - P01CA71932/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (6'-sulfated sialyl Lewis x)
RN  - 0 (Antigens, CD34)
RN  - 0 (DNA, Complementary)
RN  - 0 (Lewis X Antigen)
RN  - 0 (Ligands)
RN  - 0 (Mucins)
RN  - 0 (Oligosaccharides)
RN  - 0 (Sulfates)
RN  - 126880-86-2 (L-Selectin)
RN  - EC 2.4.1.- (Fucosyltransferases)
RN  - EC 2.4.1.152 (galactoside 3-fucosyltransferase)
RN  - EC 2.8.2.- (HEV-specific LSST)
RN  - EC 2.8.2.- (Sulfotransferases)
RN  - V956696549 (Acetylglucosamine)
SB  - IM
MH  - Acetylglucosamine/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD34/*metabolism
MH  - Base Sequence
MH  - CHO Cells
MH  - Carbohydrate Conformation
MH  - Carbohydrate Sequence
MH  - Cricetinae
MH  - DNA, Complementary/isolation & purification
MH  - Endothelium, Lymphatic/*enzymology/*immunology/metabolism
MH  - Fucosyltransferases/biosynthesis/metabolism
MH  - Humans
MH  - Hyperplasia
MH  - L-Selectin/*metabolism/physiology
MH  - Lewis X Antigen/analogs & derivatives
MH  - Ligands
MH  - Mice
MH  - Mice, Inbred AKR
MH  - Molecular Sequence Data
MH  - Mucins/metabolism
MH  - Oligosaccharides/*biosynthesis/immunology/metabolism
MH  - Rheology
MH  - Sulfates/metabolism
MH  - Sulfotransferases/genetics/isolation & purification/*metabolism
MH  - Thymus Gland/enzymology/metabolism/pathology
EDAT- 1999/08/06 00:00
MHDA- 1999/08/06 00:01
CRDT- 1999/08/06 00:00
PHST- 1999/08/06 00:00 [pubmed]
PHST- 1999/08/06 00:01 [medline]
PHST- 1999/08/06 00:00 [entrez]
AID - S1074-7613(00)80083-7 [pii]
PST - ppublish
SO  - Immunity. 1999 Jul;11(1):79-89.