PMID- 10433970
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 236
IP  - 1
DP  - 1999 Aug 5
TI  - Characterization of a gene encoding a developmentally regulated winged helix
      transcription factor of the sea urchin Strongylocentrotus purpuratus.
PG  - 97-105
AB  - Spfkh1 is a Strongylocentrotus purpuratus transcription factor that contains a
      winged helix DNA binding domain. Both the gene and overlapping cDNAs encoding
      this factor have been cloned and completely sequenced. We have mapped the start
      of transcription by primer extension to a site 600 base pairs 5' to the start of 
      translation. Spfkh1 is transcribed in one open reading frame that contains the
      DNA binding domain, nuclear localization signal and transactivation domain. The
      deduced amino acid sequence encodes a 40. 7kDa protein with a pI of 9.96.
      Alignments of the DNA binding domain with other forkhead domains reveal that this
      gene falls into Class II of the winged helix transcription factors. We have
      identified a unique carboxyl-terminal motif of unknown function that is present
      in all winged helix Class II transcription factors. A phylogenetic analysis of
      the DNA binding domains shows that, within the Class II, Spfkh1 groups with the
      deuterostomes as opposed to the protostomes. Analysis of the sequence 5' to the
      start of translation revealed binding sites for a large number of different
      transcription factors, many of which are present in multiple copies. The
      constellation of binding sites in the cis-regulatory region indicates that Spfkh1
      is regulated by a complex set of factors, some of which are known to be endoderm 
      specific. Included among these are binding sites for factors downstream of the
      Wnt/beta-catenin and hedgehog signaling pathways, implicating these pathways in
      both regulation of Spfkh1 and specification of endoderm.
FAU - David, E S
AU  - David ES
AD  - Department of Cell Biology and Biophysics, School of Biological Sciences,
      University of Missouri-Kansas City, Kansas City MO 64110, USA.
FAU - Luke, N H
AU  - Luke NH
FAU - Livingston, B T
AU  - Livingston BT
LA  - eng
SI  - GENBANK/AF149706
SI  - GENBANK/AF149707
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Fkh1 protein, Strongylocentrotus purpuratus)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (ci protein, Drosophila)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Drosophila Proteins
MH  - Forkhead Transcription Factors
MH  - Gene Expression Regulation, Developmental
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/genetics
MH  - Phylogeny
MH  - Sea Urchins/*embryology/*genetics
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction
MH  - Transcription Factors/*genetics
EDAT- 1999/08/06 00:00
MHDA- 1999/08/06 00:01
CRDT- 1999/08/06 00:00
PHST- 1999/08/06 00:00 [pubmed]
PHST- 1999/08/06 00:01 [medline]
PHST- 1999/08/06 00:00 [entrez]
AID - S0378-1119(99)00248-6 [pii]
AID - 10.1016/s0378-1119(99)00248-6 [doi]
PST - ppublish
SO  - Gene. 1999 Aug 5;236(1):97-105. doi: 10.1016/s0378-1119(99)00248-6.