PMID- 10433969
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 236
IP  - 1
DP  - 1999 Aug 5
TI  - Cloning, expression and chromosome locations of the human DNMT3 gene family.
PG  - 87-95
AB  - DNA methylation plays an important role in animal development and gene
      regulation. In mammals, several genes encoding DNA cytosine methyltransferases
      have been identified. DNMT1 is constitutively expressed and is required for the
      maintenance of global methylation after DNA replication. In contrast, the murine 
      Dnmt3 family genes appear to be developmentally regulated and behave like de novo
      DNA methyltransferases in vitro. In this study, we have cloned human DNMT3A and
      DNMT3B that encode full-length DNMT3A and DNMT3B proteins with 98% and 94% amino 
      acid sequence identity to their murine homologues. The DNMT3A and DNMT3B show
      high homology in the carboxy terminal catalytic domain and contain a conserved
      cysteine-rich region, which shares homology with the X-linked ATRX gene of the
      SNF2/SWI family. We have mapped human DNMT3A and DNMT3B to chromosomes 2p23 and
      20q11.2 respectively, and determined the DNMT3B genomic structure. We further
      show that DNMT3A expression is ubiquitous and can be readily detected in most
      adult tissues, whereas DNMT3B is expressed at very low levels in most tissues
      except testis, thyroid and bone marrow. Significantly, both DNMT3A and DNMT3B
      expression is elevated in several tumor cell lines to levels comparable to DNMT1.
      The cloning of the human DNMT3 genes will facilitate further biochemical and
      genetic studies of their functions in establishment of DNA methylation patterns, 
      regulation of gene expression and tumorigenesis.
FAU - Xie, S
AU  - Xie S
AD  - Cardiovascular Research Center, Massachusetts General Hospital, Department of
      Medicine, Harvard Medical School, Charlestown, MA 02129, USA.
FAU - Wang, Z
AU  - Wang Z
FAU - Okano, M
AU  - Okano M
FAU - Nogami, M
AU  - Nogami M
FAU - Li, Y
AU  - Li Y
FAU - He, W W
AU  - He WW
FAU - Okumura, K
AU  - Okumura K
FAU - Li, E
AU  - Li E
LA  - eng
SI  - GENBANK/AF067972
SI  - GENBANK/AF135438
GR  - GM52106/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 2.1.1.- (DNA-Cytosine Methylases)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
RN  - EC 2.1.1.37 (DNA methyltransferase 3A)
RN  - EC 2.1.1.37 (DNA methyltransferase 3B)
RN  - EC 2.1.1.37 (DNMT1 protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - CpG Islands
MH  - DNA (Cytosine-5-)-Methyltransferase 1
MH  - DNA (Cytosine-5-)-Methyltransferases/genetics
MH  - DNA, Complementary/analysis
MH  - DNA-Cytosine Methylases/*genetics
MH  - Gene Expression
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - RNA, Messenger/analysis
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - Tumor Cells, Cultured
EDAT- 1999/08/06 00:00
MHDA- 1999/08/06 00:01
CRDT- 1999/08/06 00:00
PHST- 1999/08/06 00:00 [pubmed]
PHST- 1999/08/06 00:01 [medline]
PHST- 1999/08/06 00:00 [entrez]
AID - S0378-1119(99)00252-8 [pii]
AID - 10.1016/s0378-1119(99)00252-8 [doi]
PST - ppublish
SO  - Gene. 1999 Aug 5;236(1):87-95. doi: 10.1016/s0378-1119(99)00252-8.