PMID- 10433554
OWN - NLM
STAT- MEDLINE
DCOM- 19990826
LR  - 20071115
IS  - 1044-5498 (Print)
IS  - 1044-5498 (Linking)
VI  - 18
IP  - 7
DP  - 1999 Jul
TI  - 14-3-3Gamma interacts with and is phosphorylated by multiple protein kinase C
      isoforms in PDGF-stimulated human vascular smooth muscle cells.
PG  - 555-64
AB  - It has recently been demonstrated that some members of the 14-3-3 protein family 
      play an important role in signal transduction leading to cellular proliferation. 
      We have previously shown that expression of 14-3-3gamma is induced by growth
      factors in human vascular smooth muscle cells (VSMC). In this study, we cloned
      the human homolog of 14-3-3gamma and observed many potential phosphorylation
      sites, suggesting the potential for post-translational modification. In VSMC
      treated with platelet-derived growth factor (PDGF), 14-3-3gamma protein was
      expressed and phosphorylated in an activation-dependent manner. Platelet-derived 
      growth factor-induced phosphorylation could be inhibited by phosphokinase C (PKC)
      inhibitory compounds, and 14-3-3gamma could be phosphorylated in the absence of
      PDGF by compounds that activate PKC. We also demonstrated interaction between
      14-3-3gamma and several PKC isoforms (alpha, beta, gamma, theta, and delta),
      implicating these PKC family isoforms as the kinases responsible for PDGF-induced
      14-3-3gamma phosphorylation. We found that 14-3-3gamma interacted with the signal
      transduction protein Raf-1, suggesting that 14-3-3gamma provides a link between
      this protein and PKC. Thus, 14-3-3gamma may represent a signal transduction
      protein that is regulated transcriptionally and post-transcriptionally by growth 
      factors.
FAU - Autieri, M V
AU  - Autieri MV
AD  - Department of Cardiology and Physiology, Temple University School of Medicine,
      Philadelphia, Pennsylvania 19140, USA. mautieri@unix.temple.edu
FAU - Carbone, C J
AU  - Carbone CJ
LA  - eng
SI  - GENBANK/AF142498
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (14-3-3 Proteins)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Protein Isoforms)
RN  - 0 (Proteins)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - 14-3-3 Proteins
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Humans
MH  - Molecular Sequence Data
MH  - Muscle, Smooth, Vascular/*metabolism
MH  - Phosphorylation
MH  - Platelet-Derived Growth Factor/*pharmacology
MH  - Precipitin Tests
MH  - Protein Isoforms/*metabolism
MH  - Protein Kinase C/*metabolism
MH  - Proteins/*metabolism
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Time Factors
MH  - *Tyrosine 3-Monooxygenase
EDAT- 1999/08/05 00:00
MHDA- 1999/08/05 00:01
CRDT- 1999/08/05 00:00
PHST- 1999/08/05 00:00 [pubmed]
PHST- 1999/08/05 00:01 [medline]
PHST- 1999/08/05 00:00 [entrez]
AID - 10.1089/104454999315105 [doi]
PST - ppublish
SO  - DNA Cell Biol. 1999 Jul;18(7):555-64. doi: 10.1089/104454999315105.