PMID- 10433269
OWN - NLM
STAT- MEDLINE
DCOM- 19990813
LR  - 20190822
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 23
IP  - 3
DP  - 1999 Jul
TI  - Coupling of mGluR/Homer and PSD-95 complexes by the Shank family of postsynaptic 
      density proteins.
PG  - 583-92
AB  - Shank is a recently described family of postsynaptic proteins that function as
      part of the NMDA receptor-associated PSD-95 complex (Naisbitt et al., 1999 [this 
      issue of Neuron]). Here, we report that Shank proteins also bind to Homer. Homer 
      proteins form multivalent complexes that bind proline-rich motifs in group 1
      metabotropic glutamate receptors and inositol trisphosphate receptors, thereby
      coupling these receptors in a signaling complex. A single Homer-binding site is
      identified in Shank, and Shank and Homer coimmunoprecipitate from brain and
      colocalize at postsynaptic densities. Moreover, Shank clusters mGluR5 in
      heterologous cells in the presence of Homer and mediates the coclustering of
      Homer with PSD-95/GKAP. Thus, Shank may cross-link Homer and PSD-95 complexes in 
      the PSD and play a role in the signaling mechanisms of both mGluRs and NMDA
      receptors.
FAU - Tu, J C
AU  - Tu JC
AD  - Department of Neuroscience, The Johns Hopkins University School of Medicine,
      Baltimore, Maryland 21205, USA.
FAU - Xiao, B
AU  - Xiao B
FAU - Naisbitt, S
AU  - Naisbitt S
FAU - Yuan, J P
AU  - Yuan JP
FAU - Petralia, R S
AU  - Petralia RS
FAU - Brakeman, P
AU  - Brakeman P
FAU - Doan, A
AU  - Doan A
FAU - Aakalu, V K
AU  - Aakalu VK
FAU - Lanahan, A A
AU  - Lanahan AA
FAU - Sheng, M
AU  - Sheng M
FAU - Worley, P F
AU  - Worley PF
LA  - eng
GR  - DA10309/DA/NIDA NIH HHS/United States
GR  - DA11742/DA/NIDA NIH HHS/United States
GR  - KO2 MH01152/MH/NIMH NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Calcium Channels)
RN  - 0 (Carrier Proteins)
RN  - 0 (DLGAP1 protein, human)
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (Homer Scaffolding Proteins)
RN  - 0 (ITPR1 protein, human)
RN  - 0 (Inositol 1,4,5-Trisphosphate Receptors)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Metabotropic Glutamate)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (SAP90-PSD95 Associated Proteins)
RN  - 0 (SHANK3 protein, human)
RN  - 0 (Shank1 protein, rat)
RN  - 0 (Shank3 protein, rat)
RN  - 0 (metabotropic glutamate receptor type 1)
RN  - 0 (postsynaptic density proteins)
RN  - 9DLQ4CIU6V (Proline)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Binding Sites/physiology
MH  - COS Cells
MH  - Calcium/metabolism
MH  - Calcium Channels/metabolism
MH  - Carrier Proteins/chemistry/genetics/*metabolism
MH  - Disks Large Homolog 4 Protein
MH  - Homer Scaffolding Proteins
MH  - Humans
MH  - Inositol 1,4,5-Trisphosphate Receptors
MH  - Intracellular Signaling Peptides and Proteins
MH  - Kidney/cytology
MH  - Membrane Proteins
MH  - Microscopy, Immunoelectron
MH  - Mutagenesis, Site-Directed/physiology
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurons/*chemistry/metabolism
MH  - Neuropeptides/chemistry/*metabolism
MH  - Proline/metabolism
MH  - Protein Structure, Tertiary
MH  - Rabbits
MH  - Rats
MH  - Receptors, Cytoplasmic and Nuclear/metabolism
MH  - Receptors, Metabotropic Glutamate/*metabolism
MH  - Receptors, N-Methyl-D-Aspartate/metabolism
MH  - SAP90-PSD95 Associated Proteins
MH  - Synapses/chemistry/metabolism/ultrastructure
MH  - Transfection
EDAT- 1999/08/05 00:00
MHDA- 1999/08/05 00:01
CRDT- 1999/08/05 00:00
PHST- 1999/08/05 00:00 [pubmed]
PHST- 1999/08/05 00:01 [medline]
PHST- 1999/08/05 00:00 [entrez]
AID - S0896-6273(00)80810-7 [pii]
AID - 10.1016/s0896-6273(00)80810-7 [doi]
PST - ppublish
SO  - Neuron. 1999 Jul;23(3):583-92. doi: 10.1016/s0896-6273(00)80810-7.