PMID- 10433093
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20190921
IS  - 0891-6934 (Print)
IS  - 0891-6934 (Linking)
VI  - 30
IP  - 1
DP  - 1999
TI  - Differential expression of p95vav in primary lymphoid tissue of BB rats congenic 
      for the lymphopenia gene.
PG  - 37-42
AB  - Lyp controls lymphopenia and T-cell mediated autoimmune diabetes in the
      BioBreeding (BB) rat possibly by interacting with T-cell maturation in the
      thymus. The protooncogene vav (p95) is involved in T-cell activation and in the
      intrathymic selection of developing T cells. We have previously reported
      increased production of IFN-gamma of self reactive thymocytes in the thymus
      medulla of Lyp/Lyp BB rats. Lymphopenia and diabetes may therefore be linked to
      an increase in thymocyte activation leading to a bias in thymocyte development.
      The purpose of this study was therefore to investigate whether the expression of 
      p95"a" in primary lymphoid tissues from congenic Lyp/Lyp, Lyp/+ and +/+ BB rats
      was correlated to the Lyp genotype using in situ hybridization and reverse
      transcription (RT)-PCR. It was found that the expression of vav mRNA in the
      thymus was increased in Lyp/Lyp compared to Lyp/+ and +/+ rats (p < 0.05).
      Western blot analysis revealed that the amount of p95 vav protein in Lyp/Lyp
      thymus was also increased. The results show that vav expression correlates with
      the lymphopenia phenotype and diabetes development in congenic Lyp/Lyp BB rats.
      An increase in the availability of p95vav during the development and activation
      of thymocytes in Lyp/Lyp BB rats may therefore contribute to the generation of
      islet autoreactivity, lymphopenia or both.
FAU - Bieg, S
AU  - Bieg S
AD  - Robert H. Williams Laboratory, Department of Medicine, University of Washington, 
      Seattle 98195, USA. sbieg@u.washington.edu
LA  - eng
GR  - AI42380/AI/NIAID NIH HHS/United States
GR  - DK46620/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Autoimmunity
JT  - Autoimmunity
JID - 8900070
RN  - 0 (DNA Primers)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-vav)
RN  - 0 (Vav1 protein, rat)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Animals, Congenic
MH  - Blotting, Western/veterinary
MH  - Colorimetry/veterinary
MH  - DNA Primers/chemistry
MH  - Electrophoresis, Polyacrylamide Gel/veterinary
MH  - *Gene Expression Regulation, Neoplastic
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization
MH  - Lymphopenia/genetics/immunology/veterinary
MH  - Nucleic Acid Hybridization
MH  - Oncogene Proteins/*genetics/immunology
MH  - Proto-Oncogene Proteins c-vav
MH  - RNA/chemistry
MH  - Rats
MH  - Rats, Inbred BB
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Specific Pathogen-Free Organisms
MH  - Thymus Gland/chemistry/immunology
EDAT- 1999/08/05 00:00
MHDA- 1999/08/05 00:01
CRDT- 1999/08/05 00:00
PHST- 1999/08/05 00:00 [pubmed]
PHST- 1999/08/05 00:01 [medline]
PHST- 1999/08/05 00:00 [entrez]
AID - 10.3109/08916939908994758 [doi]
PST - ppublish
SO  - Autoimmunity. 1999;30(1):37-42. doi: 10.3109/08916939908994758.