PMID- 10432400
OWN - NLM
STAT- MEDLINE
DCOM- 19990830
LR  - 20171116
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 56
IP  - 2
DP  - 1999 Aug
TI  - Prevention of crescentic glomerulonephritis by immunoneutralization of the
      fractalkine receptor CX3CR1 rapid communication.
PG  - 612-20
AB  - BACKGROUND: Fractalkine is a newly identified T-cell and monocyte/macrophage
      (Mphi) chemokine with a transmembrane domain and is a cell-surface protein on
      activated endothelium. It can mediate adhesion of cells expressing the
      fractalkine receptor CX3CR1. These unique features make fractalkine well suited
      for leukocyte recruitment in tissues with high blood flow as in the renal
      glomerulus. METHODS: Fractalkine expression in glomeruli and response of isolated
      glomerular inflammatory cells to fractalkine were studied in the Wistar-Kyoto
      (WKY) crescentic glomerulonephritis model. Antibody was used to confirm the
      proinflammatory role of fractalkine. RESULTS: Fractalkine was markedly induced in
      the endothelium of nephritic rat glomeruli, and inflammatory leukocytes
      infiltrating the glomeruli expressed increased levels of CX3CR1. Anti-CX3CR1
      antibody treatment dramatically blocked leukocyte infiltration in the glomeruli, 
      prevented crescent formation, and improved renal function. CONCLUSIONS:
      Fractalkine plays a central role in leukocyte trafficking at the endothelium in
      the high-flow glomerular circuit and, in turn, implicates CX3CR1 as a prime drug 
      target for therapeutic intervention of endothelium-related inflammatory diseases.
FAU - Feng, L
AU  - Feng L
AD  - Department of Immunology, The Scripps Research Institute, La Jolla, California
      92037, USA. llfimm@scripps.edu
FAU - Chen, S
AU  - Chen S
FAU - Garcia, G E
AU  - Garcia GE
FAU - Xia, Y
AU  - Xia Y
FAU - Siani, M A
AU  - Siani MA
FAU - Botti, P
AU  - Botti P
FAU - Wilson, C B
AU  - Wilson CB
FAU - Harrison, J K
AU  - Harrison JK
FAU - Bacon, K B
AU  - Bacon KB
LA  - eng
GR  - AR-40770/AR/NIAMS NIH HHS/United States
GR  - DK20043/DK/NIDDK NIH HHS/United States
GR  - R29 DK-49832/DK/NIDDK NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Antibodies)
RN  - 0 (Blood Proteins)
RN  - 0 (CX3C Chemokine Receptor 1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Receptors, HIV)
SB  - IM
SB  - X
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Basement Membrane/chemistry/immunology
MH  - Blood Proteins/pharmacology
MH  - CX3C Chemokine Receptor 1
MH  - Chemotaxis/immunology
MH  - Endothelium/chemistry/cytology/immunology
MH  - Gene Expression/physiology
MH  - Glomerulonephritis/*immunology/*prevention & control
MH  - Kidney Glomerulus/chemistry/cytology/immunology
MH  - Leukocytes/immunology
MH  - Neutralization Tests
MH  - RNA, Messenger/analysis
MH  - Rabbits
MH  - Rats
MH  - Rats, Inbred WKY
MH  - Receptors, Cytokine/*immunology
MH  - Receptors, HIV/*immunology
EDAT- 1999/08/05 00:00
MHDA- 1999/08/05 00:01
CRDT- 1999/08/05 00:00
PHST- 1999/08/05 00:00 [pubmed]
PHST- 1999/08/05 00:01 [medline]
PHST- 1999/08/05 00:00 [entrez]
AID - S0085-2538(15)46331-8 [pii]
AID - 10.1046/j.1523-1755.1999.00604.x [doi]
PST - ppublish
SO  - Kidney Int. 1999 Aug;56(2):612-20. doi: 10.1046/j.1523-1755.1999.00604.x.