PMID- 10432220
OWN - NLM
STAT- MEDLINE
DCOM- 19990908
LR  - 20131121
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 152
IP  - 1-2
DP  - 1999 Jun 25
TI  - Serotonin-inducible transcription of interleukin-1alpha in uterine smooth muscle 
      cells requires an AP-1 site: cloning and partial characterization of the rat
      IL-1alpha promoter.
PG  - 21-35
AB  - Interleukin-1 has been shown to contribute to infection-induced inflammatory
      processes during pregnancy. Prior work from this laboratory has demonstrated that
      serotonin-induced IL-1alpha also is required for the in-vitro production of
      collagenase in uterine smooth muscle cells, a normal, non-inflammatory process
      that occurs in-vivo during post-partum uterine involution. To understand the
      molecular mechanisms that regulate transcription of the IL-1alpha gene in these
      cells, we isolated and characterized 1.6 kilobases of the 5'-flanking region of
      the rat IL-1alpha gene. Sequencing and primer extension identified a single
      transcription start site and multiple potential regulatory elements, including a 
      TATA box at - 30 bp, a CAAT box at - 74 bp, and a conserved AP-1 site at - 9 bp. 
      This 5'-flanking DNA exhibited low basal promoter activity that was inducible by 
      serotonin. Serotonin-induced promoter activity was unaffected or induced by
      either medroxyprogesterone or IL-1 receptor antagonist. This occurred despite the
      ability of both of these hormones to markedly decrease IL-1alpha mRNA. Deletional
      analysis revealed a strong repressor in the region between - 147 and - 98 bp;
      removal of this sequence resulted in a fivefold higher basal promoter activity
      that was still serotonin responsive. Constitutive promoter activity appeared to
      reside between - 97 and - 22 bp. Deletion of this promoter region, which
      contained the TATA and CAAT boxes and an NF-IL-6/PEA-3 site, resulted in
      decreased basal transcriptional activity to the low level seen in larger
      constructs. Mutational analysis showed that serotonin-inducible transcriptional
      activity was mediated, at least in part, by the conserved AP-1 site at - 9 bp.
      This site is located within a larger extended palindromic region:
      5'-AAGCCTGACTCAGACTT-3', that together effects both the basal and
      serotonin-inducible expression of the IL-1alpha gene.
FAU - Huang, T T
AU  - Huang TT
AD  - Department of Biochemistry and Molecular Biology, Albany Medical College, NY
      12208, USA.
FAU - Vinci, J M
AU  - Vinci JM
FAU - Lan, L
AU  - Lan L
FAU - Jeffrey, J J
AU  - Jeffrey JJ
FAU - Wilcox, B D
AU  - Wilcox BD
LA  - eng
SI  - GENBANK/AF016188
GR  - HD 05291/HD/NICHD NIH HHS/United States
GR  - HD 32585/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Interleukin-1)
RN  - 0 (Transcription Factor AP-1)
RN  - 333DO1RDJY (Serotonin)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA Mutational Analysis
MH  - Female
MH  - Free Radical Scavengers/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Interleukin-1/biosynthesis/*genetics
MH  - Molecular Sequence Data
MH  - Muscle, Smooth/*physiology
MH  - Pregnancy
MH  - *Promoter Regions, Genetic
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sequence Alignment
MH  - Serotonin/*pharmacology
MH  - Transcription Factor AP-1/*genetics
MH  - Transcription, Genetic/drug effects
MH  - Uterus/physiology
EDAT- 1999/08/04 00:00
MHDA- 1999/08/04 00:01
CRDT- 1999/08/04 00:00
PHST- 1999/08/04 00:00 [pubmed]
PHST- 1999/08/04 00:01 [medline]
PHST- 1999/08/04 00:00 [entrez]
AID - S0303-7207(99)00067-2 [pii]
PST - ppublish
SO  - Mol Cell Endocrinol. 1999 Jun 25;152(1-2):21-35.