PMID- 10431835
OWN - NLM
STAT- MEDLINE
DCOM- 19990826
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 454
IP  - 3
DP  - 1999 Jul 9
TI  - Primary structure and expression analysis of human
      UDP-N-acetyl-glucosamine-2-epimerase/N-acetylmannosamine kinase, the bifunctional
      enzyme in neuraminic acid biosynthesis.
PG  - 341-4
AB  - N-Acetylneuraminic acid is a main constituent of glycoproteins and gangliosides. 
      In many membrane-bound receptors it is the target for external stimuli. The key
      enzyme for its biosynthesis is the bifunctional enzyme
      UDP-N-acetyl-glucosamine-2-epimerase/N-acetylmannosamine kinase, catalysing the
      first two steps of the biosynthesis in the cytosol. The rat enzyme was previously
      isolated and characterised. In this report we present the corresponding human
      cDNA sequence, compare it with the primary structure of the rodent enzyme, and
      report the analysis of its expression in different human tissues and cell lines.
FAU - Lucka, L
AU  - Lucka L
AD  - Institut fur Molekularbiologie und Biochemie, Universitatsklinikum Benjamin
      Franklin, Freie Universitat Berlin, Berlin-Dahlem, Germany.
FAU - Krause, M
AU  - Krause M
FAU - Danker, K
AU  - Danker K
FAU - Reutter, W
AU  - Reutter W
FAU - Horstkorte, R
AU  - Horstkorte R
LA  - eng
SI  - GENBANK/AJ238764
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - 0 (Escherichia coli Proteins)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.60 (N-acylmannosamine kinase)
RN  - EC 5.1.3.- (Carbohydrate Epimerases)
RN  - EC 5.1.3.14 (UDP acetylglucosamine-2-epimerase)
RN  - EC 5.1.3.14 (wecB protein, E coli)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Carbohydrate Epimerases/genetics/metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary/analysis/*genetics
MH  - *Escherichia coli Proteins
MH  - Humans
MH  - Molecular Sequence Data
MH  - N-Acetylneuraminic Acid/*biosynthesis
MH  - Phosphotransferases (Alcohol Group Acceptor)/*genetics/metabolism
MH  - Rats
MH  - Sequence Alignment
EDAT- 1999/08/04 00:00
MHDA- 1999/08/04 00:01
CRDT- 1999/08/04 00:00
PHST- 1999/08/04 00:00 [pubmed]
PHST- 1999/08/04 00:01 [medline]
PHST- 1999/08/04 00:00 [entrez]
AID - S0014-5793(99)00837-6 [pii]
AID - 10.1016/s0014-5793(99)00837-6 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Jul 9;454(3):341-4. doi: 10.1016/s0014-5793(99)00837-6.