PMID- 10431247
OWN - NLM
STAT- MEDLINE
DCOM- 19990826
LR  - 20161124
IS  - 1061-4036 (Print)
IS  - 1061-4036 (Linking)
VI  - 22
IP  - 4
DP  - 1999 Aug
TI  - RLIM inhibits functional activity of LIM homeodomain transcription factors via
      recruitment of the histone deacetylase complex.
PG  - 394-9
AB  - LIM domains are required for both inhibitory effects on LIM homeodomain
      transcription factors and synergistic transcriptional activation events. The
      inhibitory actions of the LIM domain can often be overcome by the LIM
      co-regulator known as CLIM2, LDB1 and NLI (referred to hereafter as CLIM2; refs
      2-4). The association of the CLIM cofactors with LIM domains does not, however,
      improve the DNA-binding ability of LIM homeodomain proteins, suggesting the
      action of a LIM-associated inhibitor factor. Here we present evidence that LIM
      domains are capable of binding a novel RING-H2 zinc-finger protein, Rlim (for
      RING finger LIM domain-binding protein), which acts as a negative co-regulator
      via the recruitment of the Sin3A/histone deacetylase corepressor complex. A
      corepressor function of RLIM is also suggested by in vivo studies of chick wing
      development. Overexpression of the gene Rnf12, encoding Rlim, results in
      phenotypes similar to those observed after inhibition of the LIM homeodomain
      factor LHX2, which is required for the formation of distal structures along the
      proximodistal axis, or by overexpression of dominant-negative CLIM1. We conclude 
      that Rlim is a novel corepressor that recruits histone deacetylase-containing
      complexes to the LIM domain.
FAU - Bach, I
AU  - Bach I
AD  - Howard Hughes Medical Institute, Eukaryotic Regulatory Biology Program,
      University of California, San Diego, School of Medicine, La Jolla 92093-0648,
      USA. ingolf.bach@zmnh.uni-hamburg.de
FAU - Rodriguez-Esteban, C
AU  - Rodriguez-Esteban C
FAU - Carriere, C
AU  - Carriere C
FAU - Bhushan, A
AU  - Bhushan A
FAU - Krones, A
AU  - Krones A
FAU - Rose, D W
AU  - Rose DW
FAU - Glass, C K
AU  - Glass CK
FAU - Andersen, B
AU  - Andersen B
FAU - Izpisua Belmonte, J C
AU  - Izpisua Belmonte JC
FAU - Rosenfeld, M G
AU  - Rosenfeld MG
LA  - eng
SI  - GENBANK/AF069990
SI  - GENBANK/AF069991
SI  - GENBANK/AF069992
SI  - GENBANK/AF069993
GR  - AR02080/AR/NIAMS NIH HHS/United States
GR  - AR044882/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Genet
JT  - Nature genetics
JID - 9216904
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.2.27 (Rlim protein, mouse)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - Chick Embryo
MH  - Extremities/anatomy & histology/embryology
MH  - Gene Expression Regulation, Developmental
MH  - Histone Deacetylases/*metabolism
MH  - Homeodomain Proteins/*antagonists & inhibitors
MH  - Mice
MH  - Molecular Sequence Data
MH  - Repressor Proteins/analysis/*physiology
MH  - Sequence Homology, Amino Acid
MH  - Time Factors
MH  - Tissue Distribution
MH  - Transcription Factors/*antagonists & inhibitors
MH  - Transfection
MH  - Ubiquitin-Protein Ligases
EDAT- 1999/08/04 10:00
MHDA- 2001/03/23 10:01
CRDT- 1999/08/04 10:00
PHST- 1999/08/04 10:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/08/04 10:00 [entrez]
AID - 10.1038/11970 [doi]
PST - ppublish
SO  - Nat Genet. 1999 Aug;22(4):394-9. doi: 10.1038/11970.