PMID- 10430899
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 16
DP  - 1999 Aug 3
TI  - MSE55, a Cdc42 effector protein, induces long cellular extensions in fibroblasts.
PG  - 9083-8
AB  - Cdc42 is a member of the Rho GTPase family that regulates multiple cellular
      activities, including actin polymerization, kinase-signaling activation, and cell
      polarization. MSE55 is a nonkinase CRIB (Cdc42/Rac interactive-binding)
      domain-containing molecule of unknown function. Using glutathione
      S-transferase-capture experiments, we show that MSE55 binds to Cdc42 in a
      GTP-dependent manner. MSE55 binding to Cdc42 required an intact CRIB domain,
      because a MSE55 CRIB domain mutant no longer interacted with Cdc42. To study the 
      function of MSE55 we transfected either wild-type MSE55 or a MSE55 CRIB mutant
      into mammalian cells. In Cos-7 cells, wild-type MSE55 localized at membrane
      ruffles and increased membrane actin polymerization, whereas expression of the
      MSE55 CRIB mutant showed fewer membrane ruffles. In contrast to these results,
      MSE55 induced the formation of long, actin-based protrusions in NIH 3T3 cells as 
      detected by immunofluorescence and live-cell video microscopy. MSE55-induced
      protrusion formation was blocked by expression of dominant-negative N17Cdc42, but
      not by expression of dominant-negative N17Rac. These findings indicate that MSE55
      is a Cdc42 effector protein that mediates actin cytoskeleton reorganization at
      the plasma membrane.
FAU - Burbelo, P D
AU  - Burbelo PD
AD  - Department of Biochemistry and Molecular Biology and Lombardi Cancer Center,
      Georgetown University Medical Center, Washington, DC 20007, USA.
      burbelop@medlib.georgetown.edu
FAU - Snow, D M
AU  - Snow DM
FAU - Bahou, W
AU  - Bahou W
FAU - Spiegel, S
AU  - Spiegel S
LA  - eng
SI  - GENBANK/AF098290
GR  - R01 GM043880/GM/NIGMS NIH HHS/United States
GR  - R37 GM043880/GM/NIGMS NIH HHS/United States
GR  - GM43880/GM/NIGMS NIH HHS/United States
GR  - R29-CA 77459-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Blood Proteins)
RN  - 0 (CDC42EP2 protein, human)
RN  - 0 (CDC42EP4 protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (GTP Phosphohydrolase Activators)
RN  - 0 (GTP-Binding Protein Regulators)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (cdc42 GTP-Binding Protein)
RN  - EC 3.6.5.2 (cdc42 GTP-Binding Protein, Saccharomyces cerevisiae)
SB  - IM
MH  - Amino Acid Substitution
MH  - Animals
MH  - Blood Proteins/genetics/*metabolism
MH  - COS Cells
MH  - Cell Cycle Proteins/*metabolism
MH  - Cell Membrane/physiology/ultrastructure
MH  - Fibroblasts/ultrastructure
MH  - *GTP Phosphohydrolase Activators
MH  - GTP Phosphohydrolases
MH  - GTP-Binding Protein Regulators
MH  - GTP-Binding Proteins/*metabolism
MH  - Glutathione Transferase/metabolism
MH  - Guanosine Triphosphate/metabolism
MH  - Microscopy, Video
MH  - Mutagenesis, Site-Directed
MH  - Recombinant Fusion Proteins/metabolism
MH  - Saccharomyces cerevisiae/enzymology
MH  - Transfection
MH  - *cdc42 GTP-Binding Protein
MH  - cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
PMC - PMC17736
EDAT- 1999/08/04 00:00
MHDA- 1999/08/04 00:01
CRDT- 1999/08/04 00:00
PHST- 1999/08/04 00:00 [pubmed]
PHST- 1999/08/04 00:01 [medline]
PHST- 1999/08/04 00:00 [entrez]
AID - 10.1073/pnas.96.16.9083 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9083-8. doi: 10.1073/pnas.96.16.9083.