PMID- 10430510
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 269
IP  - 2
DP  - 1999 Jul 9
TI  - No influence of presenilin1 I143T and G384A mutations on endogenous tau 
      phosphorylation in human and mouse neuroblastoma cells.
PG  - 83-6
AB  - Presenilin1 (PSEN1) 1143T and G384A mutations give rise to severe early-onset 
      Alzheimer's disease in two extensively studied Belgian families. In the present 
      study, we examined the effect of PSEN1 1143T and G384A mutations on tau 
      phosphorylation in human SH-SY5Y and mouse Neuro-2a neuroblastoma cell lines that 
      were transiently transfected with wild type (WT) or mutant PSEN1. With a 
      phosphorylation independent antibody, no alteration in the electrophoretic 
      mobility of tau was observed between wild type and mutant PSEN1 transfectants. 
      Also, densitometric analysis of Tau1 immunoreactivity, characteristic of 
      unphosphorylated tau, demonstrated no significant differences between WT and 
      mutant PSEN1 transfectants. Our data suggest that in the cellular models we used, 
      transient overexpression of 1143T and G384A mutant PSEN1 does not lead to 
      increased tau phosphorylation.
FAU - Julliams, A
AU  - Julliams A
AD  - Department of Molecular Genetics, Flanders Interuniversity Institute for 
      Biotechnology (VIB), University of Antwerp (UIA), Belgium.
FAU - Vanderhoeven, I
AU  - Vanderhoeven I
FAU - Kuhn, S
AU  - Kuhn S
FAU - Van Broeckhoven, C
AU  - Van Broeckhoven C
FAU - De Jonghe, C
AU  - De Jonghe C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Membrane Proteins)
RN  - 0 (PSEN1 protein, human)
RN  - 0 (Presenilin-1)
RN  - 0 (tau Proteins)
SB  - IM
MH  - Alzheimer Disease/*genetics/metabolism
MH  - Animals
MH  - Humans
MH  - Membrane Proteins/*genetics/metabolism
MH  - Mice
MH  - Mutation
MH  - Neuroblastoma
MH  - Phosphorylation
MH  - Presenilin-1
MH  - Tumor Cells, Cultured
MH  - tau Proteins/genetics/immunology/*metabolism
EDAT- 1999/08/03 00:00
MHDA- 1999/08/03 00:01
CRDT- 1999/08/03 00:00
PHST- 1999/08/03 00:00 [pubmed]
PHST- 1999/08/03 00:01 [medline]
PHST- 1999/08/03 00:00 [entrez]
AID - S0304394099004024 [pii]
AID - 10.1016/s0304-3940(99)00402-4 [doi]
PST - ppublish
SO  - Neurosci Lett. 1999 Jul 9;269(2):83-6. doi: 10.1016/s0304-3940(99)00402-4.