PMID- 10430081
OWN - NLM
STAT- MEDLINE
DCOM- 19990922
LR  - 20161122
IS  - 1078-0432 (Print)
IS  - 1078-0432 (Linking)
VI  - 5
IP  - 7
DP  - 1999 Jul
TI  - BRK/Sik expression in the gastrointestinal tract and in colon tumors.
PG  - 1767-77
AB  - Clones encoding the breast tumor kinase BRK were isolated from a normal human
      small intestinal cDNA library that was screened with the cDNA encoding the mouse 
      epithelial-specific tyrosine kinase Sik. Although BRK and Sik share only 80%
      amino acid sequence identity, Southern blot hybridizations confirmed that the two
      proteins are orthologues. Sik was mapped to mouse distal chromosome 2, which
      shows conservation of synteny with human chromosome 20q13.3, the location of the 
      BRK gene. BRK expression was examined in the normal gastrointestinal tract, colon
      tumor cell lines, and primary colon tumor samples. Like Sik, BRK is expressed in 
      normal epithelial cells of the gastrointestinal tract that are undergoing
      terminal differentiation. BRK expression also increased during differentiation of
      the Caco-2 colon adenocarcinoma cell line. Modest increases in BRK expression
      were detected in primary colon tumors by RNase protection, in situ hybridization,
      and immunohistochemical assays. The BRK tyrosine kinase appears to play a role in
      signal transduction in the normal gastrointestinal tract, and its overexpression 
      may be linked to the development of a variety of epithelial tumors.
FAU - Llor, X
AU  - Llor X
AD  - Department of Molecular Genetics, University of Illinois College of Medicine,
      Chicago 60607, USA.
FAU - Serfas, M S
AU  - Serfas MS
FAU - Bie, W
AU  - Bie W
FAU - Vasioukhin, V
AU  - Vasioukhin V
FAU - Polonskaia, M
AU  - Polonskaia M
FAU - Derry, J
AU  - Derry J
FAU - Abbott, C M
AU  - Abbott CM
FAU - Tyner, A L
AU  - Tyner AL
LA  - eng
GR  - R01 DK044525/DK/NIDDK NIH HHS/United States
GR  - R01 DK044525-06/DK/NIDDK NIH HHS/United States
GR  - DK44525/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for
      Cancer Research
JID - 9502500
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Neoplasm Proteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (PTK6 protein, human)
RN  - EC 2.7.10.2 (Ptk6 protein, mouse)
RN  - EC 2.7.10.2 (src-Family Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Biomarkers, Tumor/*biosynthesis
MH  - Caco-2 Cells
MH  - Chromosomes, Human, Pair 20
MH  - Colonic Neoplasms/chemistry/*metabolism
MH  - Digestive System/*metabolism
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins
MH  - Protein-Tyrosine Kinases/*biosynthesis/chemistry/genetics
MH  - Sequence Homology, Amino Acid
MH  - Tumor Cells, Cultured
MH  - src-Family Kinases/*biosynthesis/chemistry/genetics
EDAT- 1999/08/03 00:00
MHDA- 1999/08/03 00:01
CRDT- 1999/08/03 00:00
PHST- 1999/08/03 00:00 [pubmed]
PHST- 1999/08/03 00:01 [medline]
PHST- 1999/08/03 00:00 [entrez]
PST - ppublish
SO  - Clin Cancer Res. 1999 Jul;5(7):1767-77.