PMID- 10429212
OWN - NLM
STAT- MEDLINE
DCOM- 19990817
LR  - 20191210
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 263
IP  - 1
DP  - 1999 Jul
TI  - N-tail translocation in a eukaryotic polytopic membrane protein: synergy between 
      neighboring transmembrane segments.
PG  - 264-9
AB  - We have used the natural N-glycosylation site in the N-tail of cig30, a
      eukaryotic polytopic membrane protein, as a marker for N-tail translocation
      across the microsomal membrane. Analysis of C-terminally truncated cig30
      constructs reveals that the first transmembrane segment is sufficient for
      translocation of the wild-type N-tail; in contrast, in a mutant with four
      arginines introduced into the N-tail the second transmembrane segment is also
      required for efficient N-tail translocation. Our observations imply a
      non-sequential assembly mechanism in which the ultimate location of the N-tail
      relative to the membrane may depend on more than one transmembrane segment.
FAU - Monne, M
AU  - Monne M
AD  - Department of Biochemistry, Stockholm University, Stockholm, Sweden.
FAU - Gafvelin, G
AU  - Gafvelin G
FAU - Nilsson, R
AU  - Nilsson R
FAU - von Heijne, G
AU  - von Heijne G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Membrane Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.- (Fatty Acid Elongases)
SB  - IM
MH  - Acetyltransferases
MH  - Animals
MH  - Binding Sites
MH  - Dogs
MH  - Fatty Acid Elongases
MH  - Glycosylation
MH  - In Vitro Techniques
MH  - Intracellular Membranes/metabolism
MH  - Membrane Proteins/*chemistry/genetics/*metabolism
MH  - Microsomes/metabolism
MH  - Mutation
MH  - Recombinant Proteins/chemistry/genetics/metabolism
EDAT- 1999/08/03 00:00
MHDA- 1999/08/03 00:01
CRDT- 1999/08/03 00:00
PHST- 1999/08/03 00:00 [pubmed]
PHST- 1999/08/03 00:01 [medline]
PHST- 1999/08/03 00:00 [entrez]
AID - ejb498 [pii]
AID - 10.1046/j.1432-1327.1999.00498.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Jul;263(1):264-9. doi: 10.1046/j.1432-1327.1999.00498.x.